Establishment of CXCR4-small interfering RNA retrovirus vector driven by human prostate-specific antigen promoter and its biological effects on prostate cancer in vitro and in vivo.

Journal of Cancer Research and Clinical Oncology
Yue-Feng DuFu-Qing Zeng

Abstract

CXC chemokine receptor-4 (CXCR4) is closely involved in bone metastasis of prostate cancer, and CXCR4 levels are frequently increased in prostate cancer cells and tissues. In the present study, its biological effects on prostate cancer in vitro and in vivo and feasibility to be a therapy target were investigated using a RNA interfering retrovirus vector targeting CXCR4 gene driven by human prostate-specific antigen promoter (pPSA). We established a pPSA-siCXCR4 retrovirus vector and transfected prostate cancer cell PC-3m, LNCaP and breast cancer cell MCF-7, respectively. The expression of CXCR4 mRNA and protein was detected by RT-PCR and western blot, and the ability of adhesion, migration, invasion of prostate cancer cells was assessed using Transwell chamber. A metastasizing model using BALB/cA mice with human bone tissue implantation was established too, and transfected prostate cancer cells were via caudal vein. Survival time of mice suffering bone metastatic tumor as well as the weight and volume of these tumors were recorded and analyzed. The expression of CXCR4 mRNA and protein in androgen-responsive LNCaP cells was blocked by the pPSA-siCXCR4 vector, but it could not work in non androgen-responsive PC-3m cell and breast...Continue Reading

References

Aug 5, 1998·Proceedings of the National Academy of Sciences of the United States of America·Q MaT A Springer
Sep 10, 1999·Developmental Biology·K E McGrathJ Palis
Jan 5, 2000·The Journal of Clinical Investigation·D Y JoM A Moore
Sep 15, 2001·The New England Journal of Medicine·P M Murphy
Oct 10, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·H GeminderA Ben-Baruch
Mar 14, 2002·Proceedings of the National Academy of Sciences of the United States of America·Meng YangRobert M Hoffman
Jun 13, 2002·The Journal of Investigative Dermatology·Aimee S Payne, Lynn A Cornelius
Feb 18, 2003·Proceedings of the National Academy of Sciences of the United States of America·Maggie C LouieHong-Wu Chen
Dec 14, 2004·Cancer Immunology, Immunotherapy : CII·Alessandro OttaianoStefania Scala
Dec 14, 2005·Breast Cancer Research and Treatment·Ombretta SalvucciMark Basik
Dec 14, 2006·Cancer Metastasis Reviews·Jianhua WangRussell S Taichman
Feb 20, 2007·Molecular Aspects of Medicine·Massimo MasieroElena Favaro

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