Establishment of hairy root cultures by Agrobacterium rhizogenes mediated transformation of Isatis tinctoria L. For the efficient production of flavonoids and evaluation of antioxidant activities

PloS One
Qing-Yan GaiYu-Jie Fu

Abstract

In this work, Isatis tinctoria hairy root cultures (ITHRCs) were established as an alternative source for flavonoids (FL) production. I. tinctoria hairy root line V was found to be the most efficient line and was further confirmed by the PCR amplification of rolB, rolC and aux1 genes. Culture parameters of ITHRCs were optimized by Box-Behnken design (BBD), and eight bioactive FL constituents (rutin, neohesperidin, buddleoside, liquiritigenin, quercetin, isorhamnetin, kaempferol and isoliquiritigenin) were quali-quantitatively determined by LC-MS/MS. Under optimal conditions, the total FL accumulation of ITHRCs (24 day-old) achieved was 438.10 μg/g dry weight (DW), which exhibited significant superiority as against that of 2 year-old field grown roots (341.73 μg/g DW). Additionally, in vitro antioxidant assays demonstrated that ITHRCs extracts exhibited better antioxidant activities with lower IC₅₀ values (0.41 and 0.39, mg/mL) as compared to those of field grown roots (0.56 and 0.48, mg/mL). To the best of our knowledge, this is the first report describing FL production and antioxidant activities from ITHRCs.

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Citations

Feb 13, 2016·Frontiers in Plant Science·Christelle M AndreGea Guerriero
Jun 24, 2017·Preparative Biochemistry & Biotechnology·Kateryna O DrobotVolodymyr P Duplij
Jan 10, 2019·Preparative Biochemistry & Biotechnology·Nadiia MatvieievaJan Brindza
Nov 24, 2020·Applied Microbiology and Biotechnology·Saikat Gantait, Eashan Mukherjee

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Methods Mentioned

BETA
transgenic
PCR
electrophoresis

Software Mentioned

SPSS
Design
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