Abstract
Gentiana scabra is commonly known as 'Longdan' is an important herb in traditional Chinese medicines, commonly used for the treatment of inflammation, anorexia, indigestion and gastric infections. Iridoids and secoiridoids are main bioactive compounds which attributed to the pharmacological properties of this plant. The use of hairy root cultures as an excellent alternative for the production of pharmaceutically important metabolites in less time period with ensured quality of raw materials. An efficient hairy root culture system of Gentiana scabra and influence of different plant growth regulators (PGRs) on the production of gentiopicroside, swertiamarin and loganic acid constituents were described. Leaf explants were infected with Agrobacterium rhizogenes, which induced hairy roots up to 21%. The transformed hairy root lines were confirmed by PCR using rolB and rolC gene-specific primers. Among various solid and liquid media, B5 liquid medium resulted maximum root biomass (36- fold higher) in 4-weeks. Quantitative analysis showed loganic acid was 6.6- fold higher in the presence of zeatin (1 mg/l) and gentiopicroside accumulation was 1.8- fold higher in the presence of naphthaleneacetic acid (NAA, 1 mg/l), as compared to the ...Continue Reading
References
Apr 1, 1968·Experimental Cell Research·O L GamborgK Ojima
Jun 6, 2003·Bioscience, Biotechnology, and Biochemistry·Masahiko InoguchiHirokiyo Kondo
Oct 11, 2003·Biotechnology Advances·A Giri, M L Narasu
Apr 10, 2004·Journal of Experimental Botany·Ophélie FliniauxMarc-André Fliniaux
May 11, 2004·Biological & Pharmaceutical Bulletin·Daisuke WashidaSusumu Kitanaka
Jan 12, 2005·Life Sciences·Ekrem SezikShigeru Ito
Sep 15, 2006·Plant Cell Reports·Rajesh Kumar TiwariGuo-Chang Zheng
Apr 25, 2008·Biotechnology Advances·Victor P Bulgakov
Aug 21, 2008·Journal of Integrative Plant Biology·Hosakatte N MurthyKee Y Paek
Nov 6, 2010·Journal of Pharmaceutical and Biomedical Analysis·Anita AberhamHermann Stuppner
May 29, 2012·Biotechnology Letters·Agata Wilczańska-BarskaMirosława Krauze-Baranowska
Jun 29, 2012·Bioscience, Biotechnology, and Biochemistry·Liangbin ZhouRongmin Yu
Mar 5, 2013·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Bilal A WaniAjai P Gupta
Jan 1, 1988·Plant Cell Reports·J Mugnier
Citations
Apr 29, 2020·Plants·Dai Minh CaoPhuong Ngo Diem Quach
Jan 10, 2019·Preparative Biochemistry & Biotechnology·Nadiia MatvieievaJan Brindza
Aug 4, 2018·3 Biotech·Parisa Tavassoli, Akbar Safipour Afshar
Nov 2, 2016·Preparative Biochemistry & Biotechnology·Ł KuźmaH Wysokińska
Dec 1, 2015·Botanical Studies·Yu PanHeng-Yu Huang
Sep 13, 2019·Biotechnology Progress·Mina BeigmohamadiAli Sharafi
Nov 24, 2020·Applied Microbiology and Biotechnology·Saikat Gantait, Eashan Mukherjee
Apr 17, 2021·Frontiers in Plant Science·Sundararajan BalasubramaniR Babu Rajendran
May 12, 2021·Biotechnology Advances·Ipek SüntarSolomon Habtemariam
Jun 3, 2021·Cells·Tomasz KowalczykPrzemysław Sitarek