Establishment of heteroploid cell lines from mouse peritoneal exudate cells.

In Vitro
W R TolbertJ P Concannon

Abstract

Proliferation was observed during in vitro cultivation of peritoneal exudate cells that had been educed from a C3H mouse with Freund's incomplete adjuvant. These cells were successfully subcultured by release with trypsin-EDTA solution and are now at passage 108 after 22 months in culture. Using this technique, 12 other rapidly growing peritoneal exudate cultures were obtained, whereas 10 cultures not educed with adjuvant did not proliferate. Characteristics of four adjuvant-induced cell lines established in culture include: rapid attachment to glass, doubling time in culture of 18 to 19 hr, phagocytosis of colloidal carbon, enhanced phagocytosis of specifically sensitized bacteria, epithelium-like morphology, and retention of C3H histocompatible specificities. These cell lines had widely varying chromosome distributions with modes from 37.3 +/- 2.4 to 82.6 +/- 2.30, but inoculation of 10(7) cultured cells into syngeneic animals did not produce tumors. Procedures described for the reproducible establishment of peritoneal exudate cell lines did not require use of conditioned media or exogenous viral infection.

References

Nov 1, 1970·Journal of Virology·L B Stone, K K Takemoto
Aug 1, 1971·The Journal of Experimental Medicine·J Mauel, V Defendi
Nov 1, 1973·The Journal of General Virology·L Mallucci, J Taylor-Papadimitriou
Jul 1, 1972·Physiological Reviews·M Potter
Sep 21, 1973·Nature·S C Soderland, Y Naum
Jun 1, 1974·Journal of Cellular Physiology·H S Lin, C C Stewart
Aug 1, 1972·The Journal of Experimental Medicine·H S ShinM K Gately
Jun 1, 1972·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·J F Morgan, C P Eng

❮ Previous
Next ❯

Citations

Jan 1, 1986·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·W H de JongE J Ruitenberg

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.