Establishment of high efficiency genetic transformation system of maize mediated by Agrobacterium tumefaciens

Yi chuan = Hereditas
Kai-Fa WEI

Abstract

In order to establish high-frequency regeneration and high-efficiency genetic transformation system in maize, the significance of the 11 factors influencing maize embryonic callus induction and 9 factors affecting embryonic callus differentiation was researched by orthogonal experiment. The results showed that genotype had highly significant impact on induction of embryonic callus. The concentration of 6-BA, AgNO3, 2,4-D, ABA, and medium are the significant factors. The Multi-comparison showed that ABA 2 mg/L has a significant influence. Among the callus differentiation factors, the genotype and 6-BA concentration showed a strong main effect, the concentrations of NAA, medium, KT and 2,4-D had significant impacts on callus differentiation. Southern blotting analysis demonstrated that the resistant callus rate under the selection pressure of 25 mg/L hygromycin was a reliable indicator for system optimization in resistance screening. The concentration of acetosyringone (AS) showed sensitive differences among genotypes. The highest transformation rate was found with the optimized combination of 24-25 degrees C for co-culture temperature, 0.7 ODx15 min for Agrobacterium tumefa-ciens concentration and incubation-time, and pH 5.5-6.2...Continue Reading

References

Aug 23, 1996·Science·K J FullnerE W Nester
Jun 1, 2000·Molecular Plant-microbe Interactions : MPMI·G Hansen
Sep 27, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·W ZhangL Gilbertson
Feb 13, 2004·Proceedings of the National Academy of Sciences of the United States of America·Huixia ShouKan Wang
Apr 10, 2004·Journal of Experimental Botany·Huixia ShouKan Wang
Oct 28, 2005·Plant Cell Reports·Vladimir SidorovDavid Duncan
Dec 7, 2006·Plant Biotechnology Journal·Elizabeth E HoodJohn A Howard
Dec 16, 2006·Plant Biotechnology Journal·Barry J LamphearJohn A Howard
Feb 21, 2007·Plant Biotechnology Journal·Ashok Kumar Shrawat, Horst Lörz
Mar 3, 2007·Journal of Experimental Botany·Lisheng Kong, Patrick von Aderkas
Jun 23, 2007·Nature Protocols·Yuji IshidaToshihiko Komari
Aug 21, 2007·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Yu WangGuangtang Pan
Aug 30, 2008·Journal of Experimental Botany·Maneesha AluruSteve Rodermel

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