Establishment of human trophoblast stem cells from human induced pluripotent stem cell-derived cystic cells under micromesh culture

Stem Cell Research & Therapy
Zhuosi LiHiroo Iwata

Abstract

Trophoblasts as a specific cell lineage are crucial for the correct function of the placenta. Human trophoblast stem cells (hTSCs) are a proliferative population that can differentiate into syncytiotrophoblasts and extravillous cytotrophoblasts. Many studies have reported that chemical supplements induce the differentiation of trophoblasts from human induced pluripotent stem cells (hiPSCs). However, there have been no reports of the establishment of proliferative hTSCs from hiPSCs. Our previous report showed that culturing hiPSCs on micromesh as a bioscaffold induced cystic cells with trophoblast properties. Here, we aimed to establish hTSCs from hiPSCs. We used the micromesh culture technique to induce hiPSC differentiation into trophoblast cysts. We then reseeded and purified cystic cells. The cells derived from the reseeded cysts were highly proliferative. Low expression levels of pluripotency genes and high expression levels of TSC-specific genes were detected in proliferative cells. The cells could be passaged, and further directional differentiation into syncytiotrophoblast- and extravillous cytotrophoblast-like cells was confirmed by marker expression and hormone secretion. We established hiPSC-derived hTSCs, which may b...Continue Reading

References

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Citations

Jan 27, 2021·Journal of Oral Biosciences·Airi Tanai, Hirohiko Okamura
Mar 18, 2020·Biochemical and Biophysical Research Communications·Zhuosi LiHiroo Iwata
Apr 27, 2021·Biology of Reproduction·J ZhouD J Schust
Jul 3, 2021·Frontiers in Cell and Developmental Biology·Adam JaremekStephen J Renaud

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Methods Mentioned

BETA
ELISA
enzyme-linked immunosorbent assay
Chip
chips
ELISAs

Software Mentioned

SPSS Statistics
3D
SH800
Gene extraction

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