Establishment of long-term in vitro cultures of human ovarian cystadenomas and LMP tumors and examination of their spectrum of expression of matrix-degrading proteinases

Gynecologic Oncology
M P LuoL Dubeau

Abstract

To obtain long-term cultures of ovarian cystadenomas and ovarian tumors of low malignant potential (LMP) displaying gene expression patterns similar to those found in vivo and test the hypothesis that such cultures would express different levels of matrix-degrading proteinases than cultured ovarian carcinomas. Transfection with an adenoviral expression vector for simian virus 40 (SV40) large T antigen was used to establish long-term cultures of the above tumors. Levels of expression of various genes were evaluated using molecular biological and immunohistochemical approaches. Zymography and reverse zymography were used to examine the activity of various metalloproteinases and plasminogen activators (PA). Two-sided P values for differences in plasminogen activator expression between different cell types were evaluated by Fisher's exact test. Long-term cultures derived from cystadenomas and LMP tumors were obtained which formed colonies on semisolid supports, but were not tumorigenic in nude mice. The cultured cells expressed keratin, estrogen receptor, gonadotropin receptors, BRCA1, and originated from monoclonal populations. There was no apparent association between the malignant phenotype and the expression of either matrix me...Continue Reading

Citations

Oct 30, 2013·Annals of Oncology : Official Journal of the European Society for Medical Oncology·L Dubeau, R Drapkin
Jul 14, 2012·PloS One·Chunjing BianXiaochun Yu
Jan 5, 2002·International Journal of Cancer. Journal International Du Cancer·Jun WangWenxin Zheng
Jul 7, 2018·International Journal of Cancer. Journal International Du Cancer·Theresa AustriaLouis Dubeau
Jan 26, 2008·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·M M M WooN Auersperg
May 23, 2006·The Journal of Biological Chemistry·Hiroshi HondaPatrice J Morin
Jul 28, 2011·International Journal of Cancer. Journal International Du Cancer·Vanessa M YuLouis Dubeau
Jun 22, 2014·Cancer Prevention Research·Christine GruessnerSetsuko K Chambers

❮ Previous
Next ❯

Related Concepts

Related Feeds

Breast Cancer: BRCA1 & BRCA2

Mutations involving BRCA1, found on chromosome 17, and BRCA2, found on chromosome 13, increase the risk for specific cancers, such as breast cancer. Discover the last research on breast cancer BRCA1 and BRCA2 here.