Establishment of long-term myogenic cultures from patients with Duchenne muscular dystrophy by retroviral transduction of a temperature-sensitive SV40 large T antigen

Experimental Cell Research
L V SimonI Olsen

Abstract

We have established long-term human myogenic cultures from adult human skeletal muscle biopsies by infecting primary explant cultures with an amphotropic retroviral construct encoding a temperature-sensitive SV40 large T antigen, tsA58-U19. Infected myoblasts expressed the large T antigen and showed greatly enhanced proliferative capacity when cultured at 33 degrees C, compared with noninfected cells. When the infected cultures were incubated at 39 degrees C, the cells withdrew from cycle, aligned, and fused to form multinucleated myotubes which expressed certain antigens that are similarly expressed in nontransduced differentiating muscle cells. Myogenic clones with greatly increased proliferative capacity were generated, for the first time, from biopsies obtained from Duchenne muscular dystrophy patients as well as from normal, dystrophin-positive individuals. Cell lines produced by this approach may prove valuable for in vitro studies of myogenesis and for investigating the cellular and molecular consequences of inherited muscle diseases.

Citations

Jun 23, 2000·The Journal of Clinical Investigation·G Cossu, F Mavilio
Aug 22, 2006·Biochemical and Biophysical Research Communications·Naohiro HashimotoMasayo Inagawa
Jan 20, 2007·Molecular Therapy : the Journal of the American Society of Gene Therapy·Simon P QuennevilleJacques P Tremblay
Mar 3, 2004·Journal of Cellular Biochemistry·Peter C D MacphersonDaniel Goldman
Mar 17, 2001·Nature Reviews. Molecular Cell Biology·J R Masters
Nov 19, 2010·Regenerative Medicine·Nanette JoyceJan A Nolta
Oct 19, 2000·Equine Veterinary Journal·P R Shepherd
Nov 14, 2000·The American Journal of Pathology·S P YangP J Winyard

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