Establishment of molecular genetic approaches to study gene expression and function in an invasive hemipteran, Halyomorpha halys

EvoDevo
Yong LuLeslie Pick

Abstract

Hemiptera is a large clade of insects understudied in terms of developmental biology. Halyomorpha halys, the Brown Marmorated Stink Bug (BMSB, referred to throughout as H. halys), is an invasive hemipteran pest of the mid-Atlantic region of the USA that has rapidly spread to other regions in recent years, devastating a wide range of crops using a piercing and sucking mechanism. Its phylogenetic position, polyphagous habits, and rapid spread in the USA suggested that H. halys would be an ideal system to broaden our knowledge of developmental mechanisms in insects. We and others previously generated transcriptome sequences from different life stages of this insect. Here, we describe tools to examine gene expression patterns in whole-mount H. halys embryos and to test the response of H. halys to RNA interference (RNAi). We show that spatial and temporal patterns of gene expression in H. halys can be effectively monitored by both immunostaining and in situ hybridization. We also show that delivery of dsRNA to adult females knocks down gene function in offspring, using the homeotic gene Sex combs reduced (Scr). Knockdown of Hh-Scr resulted in dramatic malformations of the mouthparts, demonstrating for the first time that RNAi is eff...Continue Reading

References

Jan 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·C C HuiY Suzuki
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Jan 1, 1985·Cold Spring Harbor Symposia on Quantitative Biology·P H O'FarrellD Wright
Jan 1, 1985·Cold Spring Harbor Symposia on Quantitative Biology·W J Gehring
Feb 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·T Kornberg
Jan 1, 1994·Developmental Genetics·S J BrownR E Denell
Mar 21, 2001·Evolution & Development·C L Hughes, T C Kaufman
Dec 1, 2001·Annual Review of Entomology·Gregory K Davis, Nipam H Patel
Feb 13, 2002·Current Biology : CB·Gregor BucherMartin Klingler
Mar 11, 2004·Evolution & Development·Ariel D ChipmanMichael Akam
Dec 13, 2005·Evolution & Development·Wim G M DamenNikola-Michael Prpic
Dec 13, 2005·Evolution & Development·Paul Z Liu, Thomas C Kaufman
Apr 14, 2006·Proceedings of the National Academy of Sciences of the United States of America·Chong Pyo ChoeSusan J Brown
Jun 23, 2006·Proceedings. Biological Sciences·Andrew D PeelMichael Akam
Mar 7, 2007·Nature Reviews. Genetics·Ronald A Jenner, Matthew A Wills
Mar 26, 2008·Nature·UNKNOWN Tribolium Genome Sequencing ConsortiumGregor Bucher
Jun 3, 2008·Trends in Genetics : TIG·Arhat AbzhanovAntonia Monteiro
Apr 17, 2009·Nature Reviews. Genetics·Ralf J Sommer
Feb 12, 2010·Cold Spring Harbor Protocols·Susan J BrownMartin Klingler
Mar 26, 2010·The International Journal of Developmental Biology·Karla D PassalacquaAleksandar Popadic
Sep 9, 2010·Annual Review of Entomology·Christiane Weirauch, Randall T Schuh
Jan 1, 2008·CSH Protocols·Kevin Pang, Mark Q Martindale
Jan 24, 2013·Proceedings of the National Academy of Sciences of the United States of America·Alison HefferLeslie Pick
Mar 1, 2012·Wiley Interdisciplinary Reviews. Developmental Biology·Linda Z Holland, Takayuki Onai
Jul 3, 2013·Developmental Biology·Jack Green, Michael Akam
Mar 22, 2014·Genetics·Stephanie E MohrNorbert Perrimon
Aug 30, 2014·BMC Genomics·Panagiotis IoannidisJulie C Dunning Hotopp
Nov 14, 2015·Genetics·Karen G HalesDavid M Roberts
Mar 5, 2016·Disease Models & Mechanisms·Berrak UgurHugo J Bellen

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Citations

Dec 14, 2019·Archives of Insect Biochemistry and Physiology·Maria RigaJohn Vontas
May 23, 2020·Archives of Insect Biochemistry and Physiology·Dhandapani GurusamySubba Reddy Palli
Aug 23, 2020·Insects·Ritesh G JainNeena Mitter
Mar 11, 2021·The Journal of Experimental Biology·Luca FinettiGiovanni Bernacchia
Apr 30, 2021·The Journal of Experimental Biology·Luca FinettiGiovanni Bernacchia

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Datasets Mentioned

BETA
GBHT01012779.1
GBHT01012041.1

Methods Mentioned

BETA
PCR
reverse transcription PCR
dissection
dissect
dissections
in
in vitro transcription
gene knockdown
ChIP

Software Mentioned

TBLASTN
BLASTN
BLAST

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