Establishment of motor neuron-V3 interneuron progenitor domain boundary in ventral spinal cord requires Groucho-mediated transcriptional corepression.

PloS One
Keith J ToddStefano Stifani

Abstract

Dorsoventral patterning of the developing spinal cord is important for the correct generation of spinal neuronal types. This process relies in part on cross-repressive interactions between specific transcription factors whose expression is regulated by Sonic hedgehog. Groucho/transducin-like Enhancer of split (TLE) proteins are transcriptional corepressors suggested to be recruited by at least certain Sonic hedgehog-controlled transcription factors to mediate the formation of spatially distinct progenitor domains within the ventral spinal cord. The aim of this study was to characterize the involvement of TLE in mechanisms regulating the establishment of the boundary between the most ventral spinal cord progenitor domains, termed pMN and p3. Because the pMN domain gives rise to somatic motor neurons while the p3 domain generates V3 interneurons, we also examined the involvement of TLE in the acquisition of these neuronal fates. A combination of in vivo loss- and gain-of-function studies in the developing chick spinal cord was performed to characterize the role of TLE in ventral progenitor domain formation. It is shown here that TLE overexpression causes increased numbers of p3 progenitors and promotes the V3 interneuron fate whi...Continue Reading

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Citations

Jun 15, 2013·Cellular and Molecular Life Sciences : CMLS·Cédric Francius, Frédéric Clotman
Dec 21, 2013·Nature Communications·Federica VerginelliStefano Stifani
Apr 11, 2015·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Ang LiCheng Ming Chuong
May 12, 2015·Nature Communications·Christophe LaumonnerieSara I Wilson
Oct 28, 2014·Frontiers in Cellular Neuroscience·Nicolas Stifani

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Methods Mentioned

BETA
PCR
coimmunoprecipitation
immunoprecipitation
transfection

Software Mentioned

Northern Eclipse
Image J
GraphPad Prism

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