Establishment of Novel Monoclonal Antibody PMab-32 Against Rabbit Podoplanin

Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
Ryusuke HonmaYukinari Kato

Abstract

Podoplanin (PDPN) is a type I transmembrane O-glycoprotein, which is known as a specific lymphatic marker. PDPN activates platelet aggregation by binding to C-type lectin-like receptor-2 (CLEC-2) on platelet. PDPN is also expressed in several normal tissues, including podocytes and type I alveolar cells. Although many monoclonal antibodies (MAbs) against human PDPN (hPDPN), mouse PDPN (mPDPN), and rat PDPN (rPDPN) have been established, useful antibodies against rabbit PDPN (rabPDPN) have not been developed. In this study, we immunized mice with the recombinant proteins of rabPDPN, and developed a novel anti-rabPDPN MAb, named PMab-32. PMab-32 could detect endogenous and exogenous rabPDPN in flow cytometry and Western blot analysis. The KD of PMab-32 was determined to be 6.2 × 10(-8) M by flow cytometry. Immunohistochemical analysis showed that PMab-32 is useful for detecting podocytes, type I alveolar cells, and lymphatic endothelial cells in normal rabbit tissues. PMab-32 is expected to be useful for various rabbit experiments.

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Citations

Mar 5, 2016·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Yukinari KatoMika Kato Kaneko
Mar 16, 2016·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Ryusuke HonmaYukinari Kato
May 6, 2016·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Satoshi OgasawaraYukinari Kato
Jun 9, 2016·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Ryusuke HonmaYukinari Kato
Jun 22, 2016·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Satoshi OgasawaraYukinari Kato
Jul 6, 2016·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Ryusuke HonmaYukinari Kato
May 13, 2017·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Shinji YamadaYukinari Kato
Jul 25, 2017·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Shinji YamadaYukinari Kato
Nov 2, 2017·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Shunsuke ItaiYukinari Kato
Dec 21, 2018·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Yukinari KatoMika K Kaneko
Dec 21, 2018·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Yukinari KatoMika K Kaneko
Apr 3, 2019·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Mika K KanekoYukinari Kato
Apr 23, 2019·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Yoshikazu FurusawaYukinari Kato
Nov 21, 2018·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Shinji YamadaYukinari Kato
Jun 5, 2019·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Shinji YamadaYukinari Kato
Jun 5, 2019·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Yukinari KatoMika K Kaneko
Jul 18, 2019·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Junko TakeiYukinari Kato
Oct 16, 2019·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Yusuke SayamaYukinari Kato
Oct 31, 2019·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Mika K KanekoYukinari Kato
Jan 15, 2020·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Yusuke SayamaYukinari Kato
Apr 3, 2020·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Yusuke SayamaYukinari Kato
May 14, 2020·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Yukinari KatoMika K Kaneko
Jul 18, 2020·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Mika K KanekoYukinari Kato
Jan 27, 2019·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Yoshikazu FurusawaYukinari Kato
Oct 27, 2018·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Shinji YamadaYukinari Kato
May 22, 2019·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Junko TakeiYukinari Kato
Aug 30, 2019·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Shinji YamadaYukinari Kato
Dec 29, 2018·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Yukinari KatoMika K Kaneko
Oct 23, 2019·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Yukinari KatoMika K Kaneko
Feb 26, 2019·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Yukinari KatoMika K Kaneko
May 1, 2020·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Yukinari KatoMika K Kaneko
Oct 27, 2018·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Yoshikazu FurusawaYukinari Kato
Aug 14, 2019·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Junko TakeiYukinari Kato
May 28, 2021·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Tomohiro TanakaMika K Kaneko
Jun 24, 2021·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Ren NanamiyaYukinari Kato

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
chip
biosensor
ELISA
flow cytometry
flow
surface plasmon resonance

Software Mentioned

BIAcore X100 Evaluation Software (
Prism
GraphPad PRISM
BIAcore

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