Establishment of primary cell culture and an intracranial xenograft model of pediatric ependymoma: a prospect for therapy development and understanding of tumor biology

Oncotarget
Lorena Favaro PavonSérgio Cavalheiro

Abstract

Ependymoma (EPN), the third most common pediatric brain tumor, is a central nervous system (CNS) malignancy originating from the walls of the ventricular system. Surgical resection followed by radiation therapy has been the primary treatment for most pediatric intracranial EPNs. Despite numerous studies into the prognostic value of histological classification, the extent of surgical resection and adjuvant radiotherapy, there have been relatively few studies into the molecular and cellular biology of EPNs. We elucidated the ultrastructure of the cultured EPN cells and characterized their profile of immunophenotypic pluripotency markers (CD133, CD90, SSEA-3, CXCR4). We established an experimental EPN model by the intracerebroventricular infusion of EPN cells labeled with multimodal iron oxide nanoparticles (MION), thereby generating a tumor and providing a clinically relevant animal model. MRI analysis was shown to be a valuable tool when combined with effective MION labeling techniques to accompany EPN growth. We demonstrated that GFAP/CD133+CD90+/CD44+ EPN cells maintained key histopathological and growth characteristics of the original patient tumor. The characterization of EPN cells and the experimental model could facilitate...Continue Reading

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Citations

Aug 23, 2019·Brain Pathology·Ashleigh Lester, Kerrie L McDonald
May 28, 2021·Cancer Cytopathology·Jinhan XieAndrew J Gifford

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Methods Mentioned

BETA
xenograft
fluorescence assay
fluorescence imaging
fluorescence microscopy
X-ray
Fluorescence
surgical resection
enzymatic dissociation
FACS
scanning electron microscopy

Software Mentioned

Paravision
multiplex
FlowJo
FACSDiva

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