Establishment of prostatic cell line "Pro9ad" from a p53-deficient mouse

The Prostate
M HanazonoY Tomooka

Abstract

We demonstrated that p53-deficiency is sufficient for immortalization of fetal uterine cells. In the present study, we further extended our previous observations to prostate tissues from a young p53-deficient adult mouse. Cell lines were established from the ventral prostate of a p53-deficient male mouse and maintained in medium containing 10% heat-inactivated fetal calf serum supplemented with insulin (10 microg/ml), transferrin (10 microg/ml), cholera toxin (10 ng/ml), and selenium (10(-8) M). Pro9ad, one of the lines established, exhibits a typical epithelial morphology in culture. Despite the possession of androgen receptors, the growth of Pro9ad was not stimulated by 5alpha-dihydrotestosterone. Hepatocyte growth factor (HGF) slightly stimulated proliferation, whereas fibroblast growth factor-1 (FGF-1), keratinocyte growth factor (KGF), and platelet-derived growth factor AB (PDGF-AB) had no stimulating effect on growth. However, FGF-2, epidermal growth factor (EGF), and insulin-like growth factor-1 (IGF-1) accelerated proliferation in a dose-dependent manner. EGF and IGF-1 additively stimulated growth. These results suggest that Pro9ad shares characteristics in common with primary prostatic epithelial cells despite p53-defi...Continue Reading

References

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Citations

Dec 25, 2009·In Vitro Cellular & Developmental Biology. Animal·Chiho TakahashiYasuhiro Tomooka
Dec 13, 2005·Brain Research·Makoto HoriuchiYasuhiro Tomooka
Aug 2, 2005·Neuroscience Research·Makoto Horiuchi, Yasuhiro Tomooka
May 24, 2003·In Vitro Cellular & Developmental Biology. Animal·Kayo TanahashiYasuhiro Tomooka
Sep 25, 2003·In Vitro Cellular & Developmental Biology. Animal·Tomohiro UmezuYasuhiro Tomooka
Jan 12, 2007·Journal of Vascular Research·Tatsuaki NishiyamaIchiro Saito
Apr 3, 2001·Journal of Cutaneous Medicine and Surgery·J A Bush, G Li

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