Establishment of Salvia castanea Diels f. tomentosa Stib. hairy root cultures and the promotion of tanshinone accumulation and gene expression with Ag⁺, methyl jasmonate, and yeast extract elicitation

Protoplasma
Bo LiYonghong Zhu

Abstract

Salvia castanea Diels f. tomentosa Stib. is an endemic medicinal plant distributed in China, and the notably high content of tanshinone IIA in the root is proven effective for the therapy of heart diseases. Hairy root induction of this Salvia species was inoculated with Agrobacterium rhizogenes strain ATCC 15834. Transformed hairy root was cultured in 6,7-V liquid medium for growth kinetics assessment and elicitation. An S curve was present in the hairy root cultures based on the fresh and dry weights with an interval of 3 days. An optimum concentration of the applied elicitors (15 μM Ag(+), 200 μM methyl jasmonate, and 200 mg l(-1) yeast extract elicitor) benefitted both the growth status and tanshinone accumulation in the hairy root cultures. Tanshinone IIA contents were mostly stimulated 1.8-fold and 1.99-fold compared with the control by Ag(+) and methyl jasmonate elicitation, respectively. Yeast extract dramatically enhanced dry mass accumulation, while it promoted cryptotanshinone content of 2.84 ± 0.33 mg g(-1) dry weight at most in the hairy root cultures. Selected elicitors diversely influenced tanshinone accumulation in the time courses of hairy root cultures within 7 days. Furthermore, transcripts of selected genes i...Continue Reading

References

Jan 1, 1975·The Journal of General Virology·G VervlietJ Schell
Apr 1, 1970·Canadian Journal of Microbiology·I A Veliky, S M Martin
Feb 13, 2001·Plant Science : an International Journal of Experimental Plant Biology·A KrólickaE L&z shtsls;ojkowska
May 4, 2001·Trends in Plant Science·J MemelinkJ W Kijne
Mar 11, 2003·Microbiology and Molecular Biology Reviews : MMBR·Stanton B Gelvin
May 16, 2003·Proceedings of the National Academy of Sciences of the United States of America·Oliver LauleMarkus Lange
Oct 11, 2003·Biotechnology Advances·A Giri, M L Narasu
Apr 26, 2005·Biotechnology Advances·Jian ZhaoRobert Verpoorte
Jun 23, 2005·Journal of Agricultural and Food Chemistry·Braulio M FragaAzucena González-Coloma
Jul 28, 2006·Zeitschrift Für Naturforschung. C, a Journal of Biosciences·Izabela GrzegorczykHalina Wysokińska
Jul 28, 2006·Trends in Biotechnology·Stéphanie GuillonPascal Gantet
Aug 5, 2006·Medicinal Research Reviews·Xihong WangKuo-Hsiung Lee
Feb 13, 2007·Applied Microbiology and Biotechnology·Milen I GeorgievThomas Bley
Mar 18, 2009·Fitoterapia·Dongfeng YangZongsuo Liang
Oct 3, 2009·Phytochemistry·Johan Memelink
Jul 22, 2010·Pharmaceutical Biology·S Rahimi AshtianiM R Bihamta
Feb 18, 2011·Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica·Jinglei Xiao, Dacheng Jiang
Sep 13, 2011·Applied Biochemistry and Biotechnology·Manoj Kumar GoelArun Kumar Kukreja

❮ Previous
Next ❯

Citations

Jul 14, 2016·Applied Microbiology and Biotechnology·Yao LuMingliang Jin
Mar 21, 2020·Frontiers in Plant Science·Noemi Gutierrez-ValdesFlorian Cardon
May 12, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Lin LiuZongsuo Liang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.