PMID: 19912778Feb 1, 1991Paper

Establishment of Schwann cell lines by oncogene transfer

Molecular and Cellular Neurosciences
T AmagaiW Ostertag

Abstract

Schwann cell cultures prepared from murine sciatic nerves (MuSN), rat sciatic nerves (RSN) and murine dorsal root ganglia (MuDRG) were transformed by the introduction of oncogenes (v-arc, c-Ha-ras, and v-mos). The transformation was carried out by infection with helper-free retroviruses containing the oncogene and the neomycin-resistant gene which were produced in psi-2 cells. After G418 selection and cloning of cells showing spindle shape morphology, six different transformed cell clones, i.e., MuSN-arc, MuDRG-arc, MuDRG-ras, MuDRG-mos, RSN-arc, and RSN-mos, were established. All of the clones were labeled with antibodies to the S-100 protein and the laminin, which are specific markers for Schwann cells. Fibronectin and vimentin were not detected in these cell clones, in contrast to fibroblasts as 3T3 and Rat-2. These cell clones have been maintained with characteristics of Schwann cells for over 18 months. When RSN-mos, one of the Schwann cell clones, and rat retinas were cocultured direct cellular interaction was demonstrated. Furthermore, by the addition of conditioned medium of the RSN-mos, a prominent activity promoting neurite outgrowth in PC12 pheochromocytoma cells was observed.

References

Jan 1, 1990·Annual Review of Neuroscience·J W Fawcett, R J Keynes
Nov 1, 1988·Experimental Neurology·E ScarpiniD E Pleasure
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S PorterR P Bunge
Mar 1, 1985·Experimental Neurology·J A Stevenson
Jan 1, 1980·Annual Review of Neuroscience·L A Greene, E M Shooter
Feb 1, 1984·The Journal of Cell Biology·A Krystosek, N W Seeds
Jan 1, 1989·The Western Journal of Medicine·V R Hentz

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