Establishment of strongly neutralizing monoclonal antibody to human interleukin-6 and its epitope analysis.

Biochemical and Biophysical Research Communications
N IdaM Kohase

Abstract

Three monoclonal antibodies against human interleukin-6 were established and characterized. One antibody was shown to strongly neutralize both the Ig-inducing and hybridoma/plasmacytoma growth activity of interleukin-6. The results of its epitope analysis using protease treated interleukin-6 and immobilized antibody indicated that this neutralizing antibody binds to a peptide corresponding to Leu151-Lys171 of interleukin-6 molecule. Further analysis using synthetic peptides showed that a shorter peptide corresponding to Ala153-Thr162 can also inhibit the binding of the antibody to interleukin-6. These results suggest that this carboxyl-terminal region plays a crucial role in interleukin-6 functions.

References

Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·K IkebuchiM Ogawa

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Citations

Jul 5, 1997·Biotechnology and Bioengineering·C M RothM L Yarmush
Jul 1, 1996·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·K MoriyamaT Yoneda
Dec 1, 1994·Protein Science : a Publication of the Protein Society·A HammacherR J Simpson
May 1, 1997·Protein Science : a Publication of the Protein Society·R J SimpsonL D Ward
May 1, 1991·International Journal of Cell Cloning·T Hirano
Jan 1, 1990·Biotherapy·T Matsuda, T Hirano
Oct 19, 1990·Journal of Immunological Methods·N IdaM Kohase
Oct 1, 1990·Immunology Today·J F Bazan
Mar 14, 1991·European Journal of Biochemistry·C NishimuraY Arata
Sep 1, 1994·Journal of Gastroenterology and Hepatology·K OkadaA Watanabe
Nov 1, 1994·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·K TsukadaY Nagamachi
Jan 1, 1997·The Journal of Pharmacy and Pharmacology·R IgarashiY Mizushima
Nov 30, 2010·European Journal of Internal Medicine·Sahar S BessaRaghda G El-Sheikh
Feb 1, 1992·Japanese Journal of Cancer Research : Gann·K TohyamaT Nakamura
Nov 30, 2006·Journal of Cellular Biochemistry·Emiko SanoNorio Naruse
Jan 15, 1994·European Journal of Biochemistry·C J MortonR S Norton
Dec 1, 1996·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·K YoshizawaN Ida

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