Establishment of the bacterial fecal community during the first month of life in Brazilian newborns.

Clinics
Kátia BrandtMagda Carneiro-Sampaio

Abstract

The establishment of the intestinal microbiota in newborns is a critical period with possible long-term consequences for human health. In this research, the development of the fecal microbiota of a group of exclusively breastfed neonates living in low socio-economic conditions in the city of São Paulo, Brazil, during the first month of life, was studied. Fecal samples were collected from ten neonates on the second, seventh, and 30(th) days after birth. One of the neonates underwent antibiotic therapy. Molecular techniques were used for analysis; DNA was extracted from the samples, and 16S rRNA libraries were sequenced and phylogenetically analyzed after construction. A real-time polymerase chain reaction (PCR) was performed on the samples taken from the 30(th) day to amplify DNA from Bifidobacterium sp. The primary phylogenetic groups identified in the samples were Escherichia and Clostridium. Staphylococcus was identified at a low rate. Bifidobacterium sp. was detected in all of the samples collected on the 30(th) day. In the child who received antibiotics, a reduction in anaerobes and Escherichia, which was associated with an overgrowth of Klebsiella, was observed throughout the experimental period. The observed pattern of Es...Continue Reading

Citations

Dec 15, 2015·Birth Defects Research. Part C, Embryo Today : Reviews·Sharon B Meropol, Amy Edwards
Mar 25, 2014·Microbial Ecology·Carla R TaddeiMarina B Martinez
Oct 27, 2015·Iranian Journal of Pediatrics·Eu Kyoung LeeJin Tack Kim
Aug 2, 2016·Current Opinion in Infectious Diseases·Fabien MagneMauricio Farfan
Jun 30, 2015·Journal of Developmental Origins of Health and Disease·A L KozyrskyjS L Bridgman
Dec 29, 2020·Frontiers in Pediatrics·Charmaine ChewMauro Batista de Morais

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Datasets Mentioned

BETA
GU229644

Methods Mentioned

BETA
PCR

Software Mentioned

BLASTn
ACE
Phylip
PRED17
Chimera Slayer
Mothur
Chao1
BioEdit
ARB

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