Establishment of toxicity thresholds in subpopulations of coelomocytes (amoebocytes vs. eleocytes) of Eisenia fetida exposed in vitro to a variety of metals: implications for biomarker measurements

Ecotoxicology
Amaia IrizarManu Soto

Abstract

Coelomocytes comprise the immune system of earthworms and due to their sensitivity responding to a wide range of pollutants have been widely used as target cells in soil ecotoxicology. Recently, in vitro assays with primary cultures of coelomocytes based in the neutral red uptake (NRU) assay have been developed as promising tools for toxicity assessment chemical in a reproducible and cost-effective manner. However, NRU showed a bimodal dose-response curve previously described after in vivo and in vitro exposure of earthworm coelomocytes to pollutants. This response could be related with alterations in the relative proportion of coelomocyte subpopulations, amoebocytes and eleocytes. Thus, the aims of the present work were, first, to establish the toxicity thresholds that could be governed by different cell-specific sensitivities of coelomocytes subpopulations against a series of metals (Cu, Cd, Pb, Ni), and second to understand the implication that coelomocyte population dynamics (eleocytes vs. amoebocytes) after exposure to pollutants can have on the viability of coelomocytes (measured by NRU assay) as biomarker of general stress in soil health assessment. Complementarily flow cytometric analyses were applied to obtain correlat...Continue Reading

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Citations

Feb 29, 2016·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·J HomaE Kolaczkowska
Jan 13, 2018·Environmental Science and Pollution Research International·Shyamasree Ghosh
Aug 24, 2019·Ecotoxicology and Environmental Safety·Enrico Mendes SaggioroFábio Veríssimo Correia
May 22, 2019·The Science of the Total Environment·Corey M GriffithCynthia K Larive
Sep 18, 2020·Ecotoxicology and Environmental Safety·Cláudio Et ParenteEnrico Mendes Saggioro

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