Establishment of Tsc2‑deficient rat embryonic stem cells

International Journal of Oncology
Yoshitaka ItoO Hino

Abstract

Tuberous sclerosis complex (TSC) is an autosomal dominant disorder caused by TSC1 or TSC2 mutations. TSC causes the development of tumors in various organs such as the brain, skin, kidney, lung, and heart. The protein complex TSC1/2 has been reported to have an inhibitory function on mammalian target of rapamycin complex 1 (mTORC1). Treatment with mammalian target of rapamycin (mTOR) inhibitors has demonstrated tumor‑reducing effects in patients with TSC but is also associated with various adverse effects. In recent years, experiments involving in vivo differentiation of pluripotent stem cells have been reported as useful in elucidating mechanisms of pathogenesis and discovering new therapeutic targets for several diseases. To reveal the molecular basis of the pathogenesis caused by the Tsc2 mutation, we derived embryonic stem cells (ESCs) from Eker rats, which have the Tsc2 mutation and develop brain lesions and renal tumors. Although several studies have reported the necessity of Tsc1 and Tsc2 regulation to maintain ESCs and hematopoietic stem cells, we successfully established not only Tsc2+/+ and Tsc2+/- ESCs but also Tsc2-/- ESCs. We confirmed that these cells express pluripotency markers and retain the ability to differen...Continue Reading

References

Apr 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·A G Knudson
Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·G R Martin
Dec 31, 1993·Cell·UNKNOWN European Chromosome 16 Tuberous Sclerosis Consortium
Aug 8, 1997·Science·M van SlegtenhorstD J Kwiatkowski
Nov 6, 1998·Science·J A ThomsonJ M Jones
Dec 23, 1998·Proceedings of the National Academy of Sciences of the United States of America·G RennebeckC L Walker
Sep 24, 1999·The Journal of Clinical Investigation·H OndaD J Kwiatkowski
Dec 1, 1999·American Journal of Human Genetics·K S AuH Northrup
Apr 1, 2000·Journal of Neuropathology and Experimental Neurology·M MizuguchiO Hino
Jul 5, 2001·Proceedings of the National Academy of Sciences of the United States of America·T KobayashiO Hino
Nov 15, 2002·Clinical Genetics·T Tucker, J M Friedman
Dec 4, 2004·Genes, Chromosomes & Cancer·Laifong LeeSandra L Dabora
Dec 31, 2004·Journal of Neuropathology and Experimental Neurology·Jennifer A ChanDavid J Kwiatkowski
Feb 16, 2007·Congenital Anomalies·Masashi Mizuguchi
Dec 11, 2007·Annals of Neurology·Susanna M I GoordenYpe Elgersma
May 23, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Lynsey MeikleDavid J Kwiatkowski
Jun 24, 2008·Nature Medicine·Dan EhningerAlcino J Silva
Aug 30, 2008·Lancet·Paolo CuratoloSergiusz Jozwiak
Dec 5, 2008·Proceedings of the National Academy of Sciences of the United States of America·Boyi GanRonald A DePinho
Dec 23, 2008·Nature·Allison D EbertClive N Svendsen
Aug 12, 2010·Cellular Reprogramming·Charles A EasleyGerald P Schatten
Mar 18, 2011·The New England Journal of Medicine·Francis X McCormackUNKNOWN MILES Trial Group
Apr 17, 2012·Cell·Mathieu Laplante, David M Sabatini
Dec 12, 2012·Scientific Reports·Masaki Kawamata, Takahiro Ochiya
May 23, 2013·PloS One·Shilpa PrabhakarXandra O Breakefield

❮ Previous
Next ❯

Citations

Dec 11, 2020·Human Genome Variation·Saba AhmadAkira Yoshii

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.