Establishment of two cell lines from human gastric scirrhous carcinoma that possess the potential to metastasize spontaneously in nude mice

Cancer Science
Kazuyoshi YanagiharaSetsuo Hirohashi

Abstract

Few experimental studies have been conducted to clarify the mechanism of development of metastasis in scirrhous carcinoma of the stomach. In the present study, we attempted to establish gastric carcinoma cell lines by incubation of cancer cells collected from the body fluids of patients with gastric cancer. At the same time, xenografting of these cells to nude mice was performed. It was found that, of the gastric carcinoma cell lines thus established, two cell lines, designated as HSC-44PE and HSC-58, formed s.c. tumors with a high infiltrative potential (often invading the lymphatics around the cancer tissue) when implanted. Metastasis to the lymph nodes and lungs was observed in 20-40% of all the animals, indicating that the two cell lines are also capable of metastasizing spontaneously. Through repeated selection, i.e., repeated cycles of removal, culture, and implantation of the HSC cancer cells from metastatic lesions, we obtained 5 subclones of HSC-44PE and HSC-58 (designated as m2509, m2615, m2792, m2917, and m2691), which, when implanted orthotopically, exhibited the following characteristics as compared to the parent cells: (1) a higher percentage take (survival), similar frequency of metastasis, shorter time to metast...Continue Reading

References

Aug 1, 1991·Japanese Journal of Cancer Research : Gann·H NakanishiS Hosoda
Sep 1, 1991·Japanese Journal of Cancer Research : Gann·H KuniyasuD S Salomon
Jun 1, 1988·Japanese Journal of Cancer Research : Gann·H LiY Tsuchihashi
Aug 15, 1994·Cancer Letters·H YokozakiE Tahara
Feb 1, 1993·Japanese Journal of Cancer Research : Gann·H NakayamaE Tahara
Aug 19, 1993·International Journal of Cancer. Journal International Du Cancer·H KuniyasuE Tahara
May 8, 1993·International Journal of Cancer. Journal International Du Cancer·K YanagiharaF Amano
Aug 26, 1998·The American Journal of Pathology·S Hirohashi
Nov 4, 2000·International Journal of Cancer. Journal International Du Cancer·Y FukudomeS Shibamoto
Mar 13, 2001·International Review of Cytology·H YokozakiE Tahara
Jan 5, 2002·Proceedings of the National Academy of Sciences of the United States of America·Yoshinori InoSetsuo Hirohashi
Nov 28, 2002·Journal of Gastroenterology and Hepatology·Shih-Pei HuangJaw-Town Lin

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Citations

Dec 1, 2006·Journal of Cancer Research and Clinical Oncology·Yasuyo TaniYasuhito Yuasa
Jun 10, 2010·Investigational New Drugs·Fumiko TaguchiFumiaki Koizumi
Jan 26, 2006·The American Journal of Surgical Pathology·Panayiotis A KyzasKonstantinos Charalabopoulos
Jan 18, 2016·Clinics and Research in Hepatology and Gastroenterology·Shigeo NoharaYoshiaki Kajiyama
Dec 24, 2015·Gastric Cancer : Official Journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association·Anna AbeAtsushi Ochiai
Apr 20, 2007·Cancer Letters·Jeong-Seok NamLalage M Wakefield
Nov 18, 2004·Cancer Science·Satoru TakemuraKosei Hirakawa
Aug 12, 2008·Cancer Science·Kazuyoshi YanagiharaKazuto Nishio
Jan 19, 2007·International Journal of Cancer. Journal International Du Cancer·Shunji MatsumuraWataru Yasui
Oct 25, 2007·International Journal of Cancer. Journal International Du Cancer·Shun-Hua JinYasuhito Yuasa
May 10, 2008·The American Journal of Pathology·Takamasa UekitaRyuichi Sakai
Oct 7, 2009·Cancer Science·Masamitsu TanakaRyuichi Sakai
Oct 31, 2009·International Journal of Cancer. Journal International Du Cancer·Kazuyoshi YanagiharaToshio Seyama
Aug 15, 2009·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Zhang ZhuangXiao Wenlin
Jun 27, 2007·The American Journal of Pathology·Masamitsu TanakaRyuichi Sakai
Jun 18, 2016·The Journal of Pathology·Hirofumi RokutanTatsuhiro Shibata
Apr 1, 2018·International Journal of Cancer. Journal International Du Cancer·Yuki KiyozumiHideo Baba
May 31, 2018·Pathobiology : Journal of Immunopathology, Molecular and Cellular Biology·Yoshifumi TakeiKazuyoshi Yanagihara

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