Establishment of Withania somnifera hairy root cultures for the production of withanolide A

Journal of Integrative Plant Biology
Hosakatte N MurthyKee Y Paek

Abstract

Withania sominifera (Indian ginseng) was transformed by Agrobacterium rhizogenes. Explants from seedling roots, stems, hypocotyls, cotyledonary nodal segments, cotyledons and young leaves were inoculated with A. rhizogenes strain R1601. Hairy (transformed) roots were induced from cotyledons and leaf explants. The transgenic status of hairy roots was confirmed by polymerase chain reaction using nptII and rolB specific primers and, subsequently, by Southern analysis for the presence of nptII and rolB genes in the genomes of transformed roots. Four clones of hairy roots were established; these differed in their morphology. The doubling time of faster growing cultures was 8-14 d with a fivefold increase in biomass after 28 d compared with cultured, non-transformed seedling roots. MS-based liquid medium was superior for the growth of transformed roots compared with other culture media evaluated (SH, LS and N6), with MS-based medium supplemented with 40 g/L sucrose being optimal for biomass production. Cultured hairy roots synthesized withanolide A, a steroidal lactone of medicinal and therapeutic value. The concentration of withanolide A in transformed roots (157.4 microg/g dry weight) was 2.7-fold more than in non-transformed cultu...Continue Reading

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Citations

Jan 9, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Przemysław SitarekTomasz Śliwiński
Sep 13, 2012·Journal of Integrative Plant Biology·Jinzhong CuiJoshua Fenn
Apr 8, 2010·Bioresource Technology·Praveen Nagella, Hosakatte Niranjana Murthy
Jan 2, 2014·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Nilanjan ChakrabortyMaitreyi Banerjee
Jul 28, 2009·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mohammad Hossein MirjaliliJavier Palazón
Feb 22, 2021·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Janine NaßThomas Efferth
Aug 10, 2021·Phytochemistry Reviews : Proceedings of the Phytochemical Society of Europe·Tanuja P GuravAshok P Giri

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