Esterase activity and interaction of human hemoglobin with diclofenac sodium: A spectroscopic and molecular docking study.

Journal of Molecular Recognition : JMR
Neeraj DohareRajan Patel

Abstract

To get an idea about the pharmacokinetics and pharmacodynamics, it is important to study the drug-protein interaction. Therefore, herein, we studied the interaction of diclofenac sodium (DIC) with human hemoglobin. The binding study of nonsteroidal antiinflammatory drug, DIC with human hemoglobin (HHB) was done by utilizing fluorescence, UV-visible, time-resolved fluorescence and far-UV circular dichroism spectroscopy (CD). Various thermodynamic parameters such as enthalpy change (ΔH), entropy change (ΔS), and Gibbs free energy change (ΔG) were also calculated. CD results showed that DIC induces secondary structure change in HHB. Fluorescence resonance energy transfer was also performed. Additionally, it was also observed that DIC inhibits the esterase-like enzymatic activity of HHB via competitive inhibition.

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Citations

Jan 19, 2021·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Sujuan ZhuBing Wang

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