Esterase from a cariogenic bacterium hydrolyzes dental resins

Acta Biomaterialia
Bo HuangY Finer

Abstract

To identify and characterize specific esterases from S. mutans with degradative activity toward methacrylate-based resin monomers. Out of several putative esterases, an esterase encoded in an Open Reading Frame as SMU_118c (The National Center for Biotechnology Information, NCBI), was found to have true hydrolase activities. SMU_118c was cloned, expressed, purified and further characterized for its respective hydrolytic activity towards ester-containing nitrophenyl substrates and the universal resin monomers bis-phenyl-glycidyl-dimethacrylate (bisGMA) and triethyleneglycol dimethacrylate (TEGDMA) at neutral (7.0) or cariogenic (5.5) pH. Mass spectrometry (MS) was used to verify the expression of SMU_118c protein in S. mutans UA159. Similar to the whole cell activity of S. mutans, SMU_118c showed the highest affinity toward para-nitrophenyl acetate (pNPA) and para-nitrophenyl butyrate (pNPB) vs. ortho-nitrophenyl butyrate (oNPB) and butyrylthiocholine iodide (BTC) (p < 0.05). The esterase retained 60% of its activity after 21 days and hydrolyzed bisGMA at a higher rate than TEGDMA at both neutral and cariogenic pH (p < 0.001), similarly to the predominant human salivary esterase degradative activity. MS confirmed that SMU_118c i...Continue Reading

Citations

Sep 25, 2018·International Endodontic Journal·M Q MarashdehY Finer
Jul 23, 2019·Journal of Dental Research·S FrukhtbeynJ Sun
Dec 22, 2019·Journal of Dental Research·S YamauchiJ Sun
Jun 8, 2019·Operative Dentistry·Off de BritoGqm Monteiro
Nov 28, 2019·Stem Cells International·Su-Min LeeHae-Hyoung Lee
Sep 21, 2020·Acta Biomaterialia·Russel GitalisYoav Finer
Mar 6, 2021·Dental Materials : Official Publication of the Academy of Dental Materials·Ana P FugolinCarmem S Pfeifer
Apr 27, 2021·Dental Materials : Official Publication of the Academy of Dental Materials·Siemon De NysKirsten L Van Landuyt
Sep 19, 2020·Dental Materials : Official Publication of the Academy of Dental Materials·Zhongni ZhangCui Huang
Oct 12, 2018·Dental Materials : Official Publication of the Academy of Dental Materials·Cameron A Stewart, Yoav Finer
Dec 3, 2021·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Dhiraj KumarRobert S Jones
Dec 23, 2021·European Journal of Oral Sciences·June BolmeTorgils Laegreid
Sep 21, 2020·Journal of Dentistry·Isadora Martini GarciaFabrício Mezzomo Collares

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