Esterification by immobilized lipase in solvent-free media: kinetic and thermodynamic arguments

Biotechnology and Bioengineering
Georgina SandovalA Marty

Abstract

The aim of this study is to characterize, in solvent-free systems (SFS), the kinetic and thermodynamic performance of batch lipase-catalyzed esterification. SFS are compared to a conventional organic solvent, n-hexane. The esterification of oleic acid with ethanol was chosen as a model reaction. The TABEK (thermodynamic activity-based enzyme kinetics) approach was used to rationally analyze kinetics. Influence of the reaction medium on final conversions was also studied. Several factors, such as initial molar ratio of substrates, reactant availability, initial water content, and quantity of immobilized enzyme, were examined. Special attention was also turned to enzyme stability and reuse after reaction, this last item being a prerequisite in the development of industrial processes. SFS proved to be almost as efficient as n-hexane from a kinetic and thermodynamic point of view and offered a better volumetric production.

References

Dec 10, 1990·FEBS Letters·W ChulalaksananukulR M Willemot
Dec 2, 1988·Biochimica Et Biophysica Acta·M GoldbergM D Legoy
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·A Zaks, A M Klibanov
Apr 1, 1999·Biotechnology and Bioengineering·S ColombiéA Marty
Jan 5, 2000·Biotechnology and Bioengineering·M V FloresA van der Padt
Jul 1, 1987·Biotechnology and Bioengineering·C LaaneC Veeger
Feb 5, 1992·Biotechnology and Bioengineering·A MartyJ S Condoret
Mar 15, 1994·Biotechnology and Bioengineering·A MartyJ S Condoret

❮ Previous
Next ❯

Citations

May 30, 2009·Journal of Industrial Microbiology & Biotechnology·Naz ChaibakhshRaja Noor Zaliha Raja Abdul Rahman
Feb 4, 2010·Journal of Oleo Science·Salina Mat RadziRaja Noor Zaliha Raja Abdul Rahman
Oct 23, 2013·Journal of Biotechnology·Tobias KulschewskiJürgen Pleiss
Nov 5, 2016·Biotechnology Progress·Jan-Hendrik GroschAntje C Spieß
Mar 31, 2004·Biotechnology and Bioengineering·Francesco SecundoMarina Lotti
May 10, 2017·Applied Biochemistry and Biotechnology·Govind V WaghmareVirendra K Rathod
Apr 2, 2021·Chembiochem : a European Journal of Chemical Biology·Yan ZhangZheng Guo

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.