PMID: 9652809Jul 4, 1998Paper

Estimate of true incomplete exchanges using fluorescence in situ hybridization with telomere probes

International Journal of Radiation Biology
H WuT C Yang

Abstract

To study the frequency of true incomplete exchanges in radiation-induced chromosome aberrations. Human lymphocytes were exposed to 2 Gy and 5 Gy of gamma-rays. Chromosome aberrations were studied using the fluorescence in situ hybridization (FISH) technique with whole chromosome-specific probes, together with human telomere probes. Chromosomes 2 and 4 were chosen in the present study. The percentage of incomplete exchanges was 27% when telomere signals were not considered. After excluding false incomplete exchanges identified by the telomere signals, the percentage of incomplete exchanges decreased to 11%. Since telomere signals appear on about 82% of the telomeres, the percentage of true incomplete exchanges should be even lower and was estimated to be 3%. This percentage was similar for chromosomes 2 and 4 and for doses of both 2 Gy and 5 Gy. The percentage of true incomplete exchanges is significantly lower in gamma-irradiated human lymphocytes than the frequencies reported in the literature.

Citations

Sep 6, 2011·Mutation Research·Megumi HadaFrancis A Cucinotta
Dec 15, 2015·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Yumiko SutoMomoki Hirai
Feb 24, 2006·Mutation Research·Alejandro D Bolzán, Martha S Bianchi
Jun 28, 2008·Radiation Research·Francis A CucinottaKerry A George
Apr 13, 2013·Radiation Research·Bradford D Loucas, Michael N Cornforth
Jan 8, 2016·Dose-response : a Publication of International Hormesis Society·B A UlshD R Boreham
Aug 10, 1999·Luminescence : the Journal of Biological and Chemical Luminescence·P E Stanley, L J Kricka
Sep 2, 2006·Environmental and Molecular Mutagenesis·María C Díaz FlaquéAlejandro D Bolzán
Apr 17, 2001·Radiation Research·B D Loucas, M N Cornforth
Oct 19, 2002·Radiation Research·M DuranteF A Cucinotta
Dec 14, 2001·Radiation Research·K GeorgeF A Cucinotta
Sep 18, 2020·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Alejandro D Bolzán
Mar 31, 2006·Journal of Radiation Research·Nailya ChaizhunusovaMadina Madieva
Jun 10, 2003·Journal of Radiation Research·Honglu WuFrancis A Cucinotta
Jan 20, 2005·Environmental and Molecular Mutagenesis·James D TuckerD Carl Freeman
Nov 6, 2015·Frontiers in Oncology·Kerry A GeorgeFrancis A Cucinotta
Jul 11, 2002·Mutation Research·G ObeM E Drets

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