The number of quantal transmitter packets (m), released from motor nerve terminals in response to a single stimulus, has been estimated from the ratio of the amplitudes of endplate currents (e.p.c.) to spontaneous miniature endplate currents (m.e.p.c.), in voltage-clamped endplates of the frog. At 6 degrees C, the average value of m at normal nerve-muscle junctions was about 300. If allowance is made for the temporal dispersion of quantal transmitter release during the e.p.c., this value is increased by about 30%. After treatment with diaminopyridine or tetraethylammonium, transmitter release in response to a nerve stimulus is greatly enhanced and values of m exceeding 10(4) are frequently found. Moreover, the duration of the e.p.c. becomes much longer than that of the m.e.p.cs. The number of packets then liberated during the e.p.c. is much larger than the number of 'active zones' of the endplate and may even exceed the total number of vesicles lined up in twin-files adjacent to the presynaptic membrane.
Synaptic vesicle exocytosis captured by quick freezing and correlated with quantal transmitter release
Post-synaptic potentiation: interaction between quanta of acetylcholine at the skeletal neuromuscular synapse
The number of transmitter molecules in a quantum: an estimate from iontophoretic application of acetylcholine at the neuromuscular synapse
Temperature-sensitive aspects of evoked and spontaneous transmitter release at the frog neuromuscular junction
Tetraethylammonium: voltage-dependent action on endplate conductance and inhibition of ligand binding to postsynaptic proteins
Action of vinblastine on the spontaneous release of acetylcholine at the frog neuromuscular junction
Correlation between nerve terminal size and transmitter release at the neuromuscular junction of the frog
Multimodal distribution of frog miniature endplate potentials in adult denervated and tadpole leg muscle
The unitary evoked potential at the frog nerve-muscle junction results from synchronous gating of fusion pores at docked vesicles
Spontaneous and evoked quantal neurotransmitter release at the neuromuscular junction of the larval housefly, Musca domestica
Effects of Anemonia sulcata toxin II on presynaptic currents and evoked transmitter release at neuromuscular junctions of the mouse
Spontaneous excitatory postsynaptic currents in crayfish neuromuscular junctions in the absence and presence of serotonin and 3,4-diaminopyridine
The effects of 4-aminopyridine and tetrodotoxin on the release of acetylcholine from rat striatal slices
Potassium channel blockade differentially affects the relative refractory period of frog afferent terminals and axons
Differential alteration of dopamine, acetylcholine, and glutamate release during anoxia and/or 3,4-diaminopyridine treatment
End plate currents with a physiological level of quantal secretion and after potentiation of the mediator release by 4-aminopyridine
Changes in the dimensions of release sites along terminal branches at amphibian neuromuscular synapses
A comparison of the effects of acute and chronic cholinesterase inactivation on spontaneous transmitter release
3,4-Diaminopyridine reverses respiratory paralysis induced by a presynaptically active snake venom and its major neurotoxin
Antagonism of botulinum toxin-induced muscle weakness by 3,4-diaminopyridine in rat phrenic nerve-hemidiaphragm preparations
Effect of 3,4-diaminopyridine on rat extensor digitorum longus muscle paralyzed by local injection of botulinum neurotoxin
Is the MEPP due to the release of one vesicle or to the simultaneous release of several vesicles at one active zone?
The action of intravenous anaesthetic and central depressant drugs on the contractures elicited by tetraethylammonium in the chick biventer cervicis muscle
Tetraethylammonium-methohexitone induced contractures and miniature end-plate potential discharge in the chick biventer cervicis muscle
The effect of temperature on the amplitude distributions of miniature endplate potentials in the mouse diaphragm
Effect of lanthanum ions on the amplitude distributions of miniature endplate potentials and on synaptic vesicles in frog neuromuscular junctions
Increase in the number of presynaptic large intramembrane particles during synaptic transmission at the Torpedo nerve-electroplaque junction
Effect of hypertonic saline on quantal size and synaptic vesicles in identified neuromuscular junction of the frog
Presynaptic effects of 4-aminopyridine and changes following a single giant impulse at the Torpedo nerve-electroplaque junction
Reversible effect of depolarization by K-propionate on sub-miniature endplate potential to bell-miniature endplate potential ratios, on miniature endplate potential frequencies and amplitudes, and on synaptic vesicle diameters and densities in frog neuromuscular junctions
Antagonistic effects of perilymphatic calcium and magnesium on the activity of single cochlear afferent neurons
A comparison of the actions of 4-aminopyridine, caffeine and quinine on the toad isolated rectus abdominis muscle
Lambert-Eaton sera reduce low-voltage and high-voltage activated Ca2+ currents in murine dorsal root ganglion neurons
The distribution of acetylcholine receptor clusters and sites of transmitter release along chick ciliary ganglion neurite-myotube contacts in culture
The effect of opiates on the terminal nerve impulse and quantal secretion from visualized amphibian nerve terminals
Single-pixel optical fluctuation analysis of calcium channel function in active zones of motor nerve terminals
Relationship between presynaptic calcium transients and postsynaptic currents at single gamma-aminobutyric acid (GABA)ergic boutons
Presynaptic K(+) channels, vesicular Ca(2+)/H (+) antiport--synaptotagmin, and acetylcholinesterase, three mechanisms cutting short the cholinergic signal at neuromuscular and nerve-electroplaque junctions
The effects of tetraethylammonium during twitch and tetanic stimulation of the phrenic nerve diaphragm preparation in the rat
Ion channel modulators that enhance acetylcholine release: potential therapies for Alzheimer's disease
Evidence for two distinct processes in the final stages of neurotransmitter release as detected by binomial analysis in calcium and strontium solutions
A further study of the neuromuscular effects of vesamicol (AH5183) and of its enantiomer specificity
Extracellular chloride replacement by isethionate induces abnormal spontaneous release of transmitter at the frog neuromuscular junction
A statistical model indicates that miniature end-plate potentials and unitary evoked end-plate potentials are composed of subunits
Asynchronous release of synaptic vesicles determines the time course of the AMPA receptor-mediated EPSC
Analysis of synaptic transmission in the neuromuscular junction using a continuum finite element model
An excess-calcium-binding-site model predicts neurotransmitter release at the neuromuscular junction
Interactions between tetraethylammonium and methohexitone at the chick neuromuscular junction. Experiments with the moving fluid electrode technique
Ultrafast and Slow Cholinergic Transmission. Different Involvement of Acetylcholinesterase Molecular Forms
Astrocytes with previous chronic exposure to amyloid β-peptide fragment 1-40 suppress excitatory synaptic transmission
Tetraethylammonium facilitates the stimulation-evoked loss of the enkephalins from the myenteric plexus of guinea-pig ileum
Presynaptic inhibitory action of a metabotropic glutamate receptor agonist on excitatory transmission in visual cortical neurons
Effects of a 4-aminopyridine in calcium movements and changes of membrane potential in pinched-off nerve terminals from rat cerebral cortex
Structural and functional correlates of synaptic transmission in the vertebrate neuromuscular junction
Selective effects of aniracetam across receptor types and forms of synaptic facilitation in hippocampus
Synaptic Vesicles Having Large Contact Areas with the Presynaptic Membrane are Preferentially Hemifused at Active Zones of Frog Neuromuscular Junctions Fixed during Synaptic Activity
Severe and progressive neurotransmitter release aberrations in familial hemiplegic migraine type 1 Cacna1a S218L knock-in mice
Effects of glycine on the crayfish neuromuscular junction. II. Release of inhibitory transmitter activated by glycine
Transmitter release is evoked with low probability predominately by calcium flux through single channel openings at the frog neuromuscular junction
Seasonal factors influence quantal transmitter release and calcium dependence at amphibian neuromuscular junctions
Gambierol Potently Increases Evoked Quantal Transmitter Release and Reverses Pre- and Post-Synaptic Blockade at Vertebrate Neuromuscular Junctions
Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.
Synthetic Genetic Array Analysis
Synthetic genetic arrays allow the systematic examination of genetic interactions. Here is the latest research focusing on synthetic genetic arrays and their analyses.
Congenital hyperinsulinism is caused by genetic mutations resulting in excess insulin secretion from beta cells of the pancreas. Here is the latest research.
Neural Activity: Imaging
Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.
Chronic Fatigue Syndrome
Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.
Epigenetic memory refers to the heritable genetic changes that are not explained by the DNA sequence. Find the latest research on epigenetic memory here.
Cell Atlas of the Human Eye
Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.
Femoral Neoplasms are bone tumors that arise in the femur. Discover the latest research on femoral neoplasms here.
STING Receptor Agonists
Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.