Estimates of the genomic mutation rate for detrimental alleles in Drosophila melanogaster

Genetics
Brian CharlesworthPatricia Pignatelli

Abstract

The net rate of mutation to deleterious but nonlethal alleles and the sizes of effects of these mutations are of great significance for many evolutionary questions. Here we describe three replicate experiments in which mutations have been accumulated on chromosome 3 of Drosophila melanogaster by means of single-male backcrosses of heterozygotes for a wild-type third chromosome. Egg-to-adult viability was assayed for nonlethal homozygous chromosomes. The rates of decline in mean and increase in variance (DM and DV, respectively) were estimated. Scaled up to the diploid whole genome, the mean DM for homozygous detrimental mutations over the three experiments was between 0.8 and 1.8%. The corresponding DV estimate was approximately 0.11%. Overall, the results suggest a lower bound estimate of at least 12% for the diploid per genome mutation rate for detrimentals. The upper bound estimates for the mean selection coefficient were between 2 and 10%, depending on the method used. Mutations with selection coefficients of at least a few percent must be the major contributors to the effects detected here and are likely to be caused mostly by transposable element insertions or indels.

References

Sep 3, 1992·Nature·D HouleB Charlesworth
Oct 1, 1980·Genetics·M J SimmonsJ D Raymond
Sep 1, 1993·The Journal of Heredity·A S Kondrashov
Dec 16, 1997·Proceedings of the National Academy of Sciences of the United States of America·S A ShabalinaA S Kondrashov
Jul 23, 1998·Genetical Research·B Charlesworth
Jan 20, 1999·Proceedings of the National Academy of Sciences of the United States of America·J D FryS V Nuzhdin
Oct 8, 1999·Genetics·P D Keightley, A Eyre-Walker
Mar 25, 2000·Science·M D AdamsJ C Venter
Aug 5, 2000·Genetics·A García-Dorado, A Caballero
Oct 13, 2000·Science·P D Keightley, A Eyre-Walker
Mar 31, 2001·Genetical Research·J D Fry
May 7, 2002·Evolution; International Journal of Organic Evolution·Frank H ShawRuth G Shaw
Jul 30, 2002·Evolution; International Journal of Organic Evolution·A CaballeroA García-Dorado
Apr 19, 2003·Genetics·James D Fry, Sergey V Nuzhdin
Apr 22, 2003·Evolution; International Journal of Organic Evolution·Peter D Keightley, Michael Lynch
Jun 17, 2003·Genetics·Aurora García-Dorado, Araceli Gallego
Aug 28, 2003·Evolution; International Journal of Organic Evolution·Ana RiveroStuart A West
Feb 6, 2004·Genome Research·Daniel L HalliganPeter D Keightley

❮ Previous
Next ❯

Citations

Jan 11, 2005·Nature Reviews. Genetics·John F Y Brookfield
Dec 7, 2006·Biology Letters·Laurence Loewe, Brian Charlesworth
Sep 3, 2010·Biology Letters·Luis-Miguel Chevin
Mar 24, 2010·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Fyodor A Kondrashov, Alexey S Kondrashov
Mar 24, 2010·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Laurence Loewe, William G Hill
Feb 26, 2009·BMC Systems Biology·Laurence Loewe
Jul 7, 2005·Genetics·Joanna L MacKenzieDaniel J Schoen
Mar 3, 2006·Genetics·Frédéric Guillaume, Nicolas Perrin
Nov 22, 2005·Genetics·Laurence LoeweVéronique Nöel
Apr 17, 2007·Genetics·Michael Turelli, Leonie C Moyle
Aug 11, 2005·Proceedings of the National Academy of Sciences of the United States of America·W TapperN E Morton
Dec 19, 2012·Nature Reviews. Genetics·Damon Lisch
Aug 19, 2014·Evolution; International Journal of Organic Evolution·Céline DevauxEmmanuelle Porcher
Jul 30, 2005·Evolution; International Journal of Organic Evolution·Floyd A ReedCharles F Aquadro
Jan 7, 2006·Evolution; International Journal of Organic Evolution·Daniel J Schoen
Jul 5, 2006·Evolution; International Journal of Organic Evolution·Guillaume Martin, Thomas Lenormand
Sep 29, 2009·Evolution; International Journal of Organic Evolution·Sébastien Gourbière, James Mallet
Dec 5, 2013·Evolution; International Journal of Organic Evolution·Emmanuelle Porcher, Russell Lande
May 16, 2014·Evolution; International Journal of Organic Evolution·Andrew D MorganNick Colegrave
Jun 4, 2014·Annals of the New York Academy of Sciences·Thomas Bataillon, Susan F Bailey
Jan 13, 2015·Proceedings of the National Academy of Sciences of the United States of America·Brian Charlesworth
May 18, 2016·BMC Evolutionary Biology·Emmanuelle Porcher, Russell Lande
Aug 6, 2010·PLoS Genetics·Michael M MagwireTrudy F C Mackay
Jun 16, 2016·Molecular Biology and Evolution·Thomas J Y KonoPeter L Morrell
Jun 3, 2016·Evolution; International Journal of Organic Evolution·Christelle FraïsseJohn J Welch
May 9, 2006·Experimental Gerontology·Robin J Mockett, Rajindar S Sohal
Aug 30, 2007·PloS One·Shripad D TuljapurkarMichael D Gurven
Sep 9, 2017·Evolution; International Journal of Organic Evolution·Susanne A KraemerNick Colegrave
May 15, 2013·G3 : Genes - Genomes - Genetics·David W HallDavid C Queller
Feb 13, 2019·G3 : Genes - Genomes - Genetics·Jim A MossmanDavid M Rand

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.