Jan 17, 2014

LINC complex branching through structurally diverse SUN-KASH 6:6 assemblies

BioRxiv : the Preprint Server for Biology
Meaghan E. JenkinsTony D. Auld

Abstract

The LINC complex mechanically couples cytoskeletal and nuclear components across the nuclear envelope to fulfil a myriad of cellular functions, including nuclear shape and positioning, hearing and meiotic chromosome movements. The canonical model of the LINC complex is of individual linear nucleocytoskeletal linkages provided by 3:3 interactions between SUN and KASH proteins. Here, we provide crystallographic and biophysical evidence that SUN-KASH is a constitutive 6:6 complex in which two SUN trimers interact back-to-back. A common SUN-KASH topology is achieved through structurally diverse 6:6 interaction mechanisms by distinct KASH proteins, including zinc-coordination by Nesprin-4. The SUN-KASH 6:6 complex is incompatible with the current model of a linear LINC complex and instead suggests the formation of a branched LINC complex network. In this model, SUN-KASH 6:6 complexes act as nodes for force distribution and integration between adjacent SUN and KASH molecules, enabling the coordinated transduction of large forces across the nuclear envelope.

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Mentioned in this Paper

Rupture
Brasiliorchis marginata
Positioning Attribute
Penthicodes pulchella
Strategy
Pyramica pulchella
Pithocarpa pulchella
Bulimina marginata
Cone Dosage Form
Phyciodes pulchella

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