Estimating the contributions of NMDA and non-NMDA currents to EPSPs in retinal ganglion cells

Visual Neuroscience
T J VelteR F Miller

Abstract

Whole-cell recordings were obtained from retinal ganglion cells of the tiger salamander (Ambystoma tigrinum) in a superfused slice preparation to evaluate contributions of NMDA (N-methyl-D-aspartate) and KA/AMPA (kainate/alpha-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid) receptors to excitatory postsynaptic potentials (EPSPs) of retinal ganglion cells. Synaptic activation of retinal ganglion cells was achieved through the use of a brief pressure pulse of hyperosmotic Ringer (Ringer + sucrose) delivered through a microelectrode visually placed in the inner plexiform layer while whole-cell recordings were obtained from adjacent cells in the ganglion cell layer. Separation of NMDA and KA/AMPA excitatory postsynaptic currents (EPSCs) was achieved through the application of the antagonists NBQX and D-AP7, while inhibitory currents were blocked by strychnine and picrotoxin. Simple addition of the two independent EPSCs showed, most often, that the sum of the KA/AMPA and NMDA currents was less than the control response, but in some cases the sum of the two currents exceeded the magnitude of the control response. Neither result was consistent with expectations based on voltage-clamp principles and the assumption that the two cur...Continue Reading

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Citations

May 8, 2014·Visual Neuroscience·Joanna D CrookDennis M Dacey
Jan 20, 2007·Cellular and Molecular Neurobiology·Lily Vitanova
Oct 28, 2003·The Journal of Physiology·Bang V Bui, Brad Fortune
Feb 17, 2006·Journal of Neurophysiology·Robert F MillerToby J Velte
Jun 10, 2000·Journal of Neurophysiology·E D Cohen

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