Estimation of fish biomass using environmental DNA.

PloS One
Teruhiko TakaharaZen'ichiro Kawabata

Abstract

Environmental DNA (eDNA) from aquatic vertebrates has recently been used to estimate the presence of a species. We hypothesized that fish release DNA into the water at a rate commensurate with their biomass. Thus, the concentration of eDNA of a target species may be used to estimate the species biomass. We developed an eDNA method to estimate the biomass of common carp (Cyprinus carpio L.) using laboratory and field experiments. In the aquarium, the concentration of eDNA changed initially, but reached an equilibrium after 6 days. Temperature had no effect on eDNA concentrations in aquaria. The concentration of eDNA was positively correlated with carp biomass in both aquaria and experimental ponds. We used this method to estimate the biomass and distribution of carp in a natural freshwater lagoon. We demonstrated that the distribution of carp eDNA concentration was explained by water temperature. Our results suggest that biomass data estimated from eDNA concentration reflects the potential distribution of common carp in the natural environment. Measuring eDNA concentration offers a non-invasive, simple, and rapid method for estimating biomass. This method could inform management plans for the conservation of ecosystems.

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Citations

Jan 16, 2014·Environmental Science & Technology·Matthew A BarnesDavid M Lodge
Dec 10, 2014·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Mikkel Winther PedersenEske Willerslev
Jan 16, 2016·Environmental Science & Technology·Jessica EichmillerPeter W Sorensen
Feb 16, 2016·Molecular Ecology·Constanze HoffmannSébastien Calvignac-Spencer
Oct 20, 2015·Molecular Ecology·Alice ValentiniTony Dejean
Aug 27, 2015·Molecular Ecology Resources·Kimiko UchiiToshifumi Minamoto
Nov 13, 2015·Environmental Science & Technology·Elvira MächlerFlorian Altermatt
Apr 29, 2015·Molecular Ecology Resources·Jessica J EichmillerPeter W Sorensen
Jun 3, 2015·Molecular Ecology Resources·Nathan T EvansDavid M Lodge
Oct 10, 2014·Environmental Science & Technology·Lucas M NathanAndrew R Mahon
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Sep 17, 2013·Molecular Ecology Resources·David S PilliodLisette P Waits
Nov 6, 2015·Molecular Ecology Resources·Elise M FurlanRichard P Duncan
Jun 4, 2014·Molecular Ecology Resources·Stephen F JaneAndrew R Whiteley
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Jun 3, 2016·Journal of the Royal Society, Interface·Arial J ShogrenDiogo Bolster
Jan 24, 2014·PloS One·Ryan P KellyLarry B Crowder
Jun 10, 2016·BMC Research Notes·Kelly E WilliamsAntoinette J Piaggio
Dec 6, 2014·PloS One·Atsushi MaruyamaToshifumi Minamoto
Sep 7, 2012·PloS One·Philip Francis ThomsenEske Willerslev
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Jul 8, 2017·PloS One·Kelly E WilliamsAntoinette J Piaggio

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Methods Mentioned

BETA
PCR
profiler

Software Mentioned

SPSS
BLAST
eBioX
Primer
SPSS Statistics

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