Abstract
This paper describes the interaction observed between human keratinocytes and xenogenic collagen in vitro modified by HCl. Human keratinocytes were cultivated for 3-10 days, on modified and control support. Their growth, morphology and interaction with support were analyzed. It was found that on both control and experimental (modified) collagen cells proliferated in a similar way. Within 3-10 days, the culture became multilayered and mature and differentiation of cells was visible. Using electron microscope elements of basal membrane interacting with support were seen. On modified support processes of cells penetrating the support are occasionally seen. By use of the immunofluorescent, cytochemical techniques was found the presence of: BP-180 (antigen), beta(4) integrin, laminin 5 and collagen IV, VII, VIIc. On the modified support the above listed elements appeared between 3 and 7 days of culture, whereas on the control between 7th and 10th days. On 10th day of culture, the presence of elements of basal membranes became less evident. Results give some hope for using xenogenic, modified collagen as support of keratinocytes culture in process of human skin engineering.
References
Nov 1, 1975·Cell·J G Rheinwald, H Green
Sep 1, 1988·Journal of Cataract and Refractive Surgery·D E Poland, H E Kaufman
Aug 1, 1982·Journal of Clinical Gastroenterology·S S Schwartz
Jan 1, 1980·Journal of Studies on Alcohol·L S Cermak
Aug 1, 1995·Journal of Biomedical Materials Research·S F BadylakC Simmons
Dec 1, 1993·The Journal of Investigative Dermatology·M L CooperR L Spielvogel
Apr 1, 1997·Biomaterials·A K SchlegelA Mehl
Apr 1, 1997·The British Journal of Dermatology·P MarrenF Wojnarowska
Apr 1, 1997·In Vitro Cellular & Developmental Biology. Animal·M BalasubramaniP K Sehgal
May 22, 1997·Proceedings. Biological Sciences·S E BlackshawJ A Davies
May 8, 1999·The Journal of Investigative Dermatology·H J StarkN E Fusenig
Aug 15, 2000·Tissue Engineering·A I Caplan
Aug 15, 2000·Tissue Engineering·R E HorchG B Stark
Sep 19, 2000·Tissue Engineering·E J Orwin, A Hubel
Jun 9, 2001·International Journal of Pharmaceutics·C H LeeY Lee
Jul 17, 2004·Cell and Tissue Banking·A KinsnerD Sladowski
Citations
Mar 4, 2011·Langenbeck's Archives of Surgery·Jörn Andreas LohmeyerHans-Günther Machens
Oct 18, 2007·Cells, Tissues, Organs·S XiaoG Wang
Aug 25, 2009·Biomaterials·G LammersT H van Kuppevelt