Estimation of lactic acid bacterial cell number by DNA quantification

Drug Discoveries & Therapeutics
Masaki IshiiKazuhisa Sekimizu

Abstract

Lactic acid bacteria are provided by fermented foods, beverages, medicines, and supplements. Because the beneficial effects of medicines and supplements containing functional lactic acid bacteria are related to the bacterial cell number, it is important to establish a simple method for estimating the total number of lactic acid bacterial cells in the products for quality control. Almost all of the lactic acid bacteria in the products are dead, however, making it difficult to estimate the total number of lactic acid bacterial cells in the products using a standard colony-counting method. Here we estimated the total lactic acid bacterial cell number in samples containing dead bacteria by quantifying the DNA. The number of viable Enterococcus faecalis 0831-07 cells decreased to less than 1 × 10-8 by 15 min of heat treatment at 80°C. The amount of extracted DNA from heat-treated cells was 78% that of non-heated cells. The number of viable Lactobacillus paraplantarum 11-1 cells decreased to 1 × 10-4 after 4 days culture. The amount of extracted DNA of the long-cultured cells, however, was maintained at 97%. These results suggest that cell number of lactic acid bacteria killed by heat-treatment or long-term culture can be estimated b...Continue Reading

References

Feb 1, 1992·Applied and Environmental Microbiology·M WhitingB Ziola
Oct 20, 2012·Journal of Industrial Microbiology & Biotechnology·Mohammad SalmaHervé Alexandre
Apr 23, 2013·Applied Microbiology and Biotechnology·Youbao ZhaoKeqian Yang
Apr 4, 2014·The American Journal of Clinical Nutrition·Lorenzo Morelli
Mar 15, 2016·Drug Discoveries & Therapeutics·Satoshi NishidaKazuhisa Sekimizu
Feb 17, 2017·Drug Discoveries & Therapeutics·Masaki IshiiKazuhisa Sekimizu
Feb 17, 2017·Drug Discoveries & Therapeutics·Masaki IshiiKazuhisa Sekimizu
Mar 28, 2018·Annual Review of Food Science and Technology·Kieran M LynchElke K Arendt

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Citations

Nov 23, 2018·Drug Discoveries & Therapeutics·Hiroto NakajimaKazuhisa Sekimizu

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