PMID: 9192634Jun 24, 1997Paper

Estimation of mean exocytic vesicle capacitance in mouse adrenal chromaffin cells

Proceedings of the National Academy of Sciences of the United States of America
Tobias Moser, E Neher

Abstract

Whole-cell membrane capacitance measurements are frequently used to monitor neuronal and nonneuronal secretory activity. However, unless individual fusion events can be resolved, the type of the fusing vesicles cannot be identified in these experiments. Here we apply statistical analysis of trial-to-trial variations between depolarization-induced capacitance increases of mouse adrenal chromaffin cells and obtain estimates for the capacitance contribution of individual exocytic vesicles between 0.6 and 2 fF. For comparison, measurements of membrane capacitance were combined with amperometric recordings of catecholamine release during intracellular perfusion of chromaffin cells with high [Ca2+]. Crosscorrelation of both signals yielded a mean capacitance contribution of individual catecholaminergic vesicles of 1.3 fF. We suggest that depolarization-induced capacitance increases in mouse adrenal chromaffin cells mainly represent fusion of chromaffin granules.

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Citations

Apr 28, 2011·Analytical and Bioanalytical Chemistry·Secil KoseogluChristy L Haynes
Jan 9, 2007·Pflügers Archiv : European journal of physiology·Andreas NeefTobias Moser
Dec 17, 2008·Pflügers Archiv : European journal of physiology·Thien An Duong DinhAndreas Lückhoff
Jul 5, 2006·Cell and Tissue Research·Ute Becherer, Jens Rettig
Nov 21, 2000·Journal of Neuroscience Methods·F SeguraR Borges
Feb 13, 2001·Journal of Neuroscience Methods·T L ColliverA G Ewing
Feb 11, 2004·The Journal of General Physiology·Matthias BraunPatrik Rorsman
Apr 1, 1999·Annual Review of Physiology·H von Gersdorff, G Matthews
Apr 2, 2014·The Journal of Cell Biology·Madhurima DharaDieter Bruns
Feb 6, 2004·Proceedings of the National Academy of Sciences of the United States of America·Laura P Zanello, Anthony W Norman
Sep 26, 2006·Advanced Drug Delivery Reviews·Haruo KasaiNoriko Takahashi
Mar 16, 2000·Reviews in the Neurosciences·D Sulzer, E N Pothos
Aug 4, 2005·Cell Calcium·Fernando D Marengo
Apr 12, 2005·Neuron·Dina SpeidelJens Rettig
Dec 18, 1998·Neuron·H von GersdorffM Tachibana
Nov 13, 1999·Nature Cell Biology·E AlésG Alvarez de Toledo
Feb 22, 2002·The Journal of Biological Chemistry·Alexander G Obukhov, Martha C Nowycky
Nov 23, 2018·Proceedings of the National Academy of Sciences of the United States of America·Chad Paul Grabner, Tobias Moser
Nov 20, 2002·Annals of the New York Academy of Sciences·J Francisco GomezRicardo Borges
Oct 4, 2006·Physiological Reviews·Antonio G GarcíaJavier García-Sancho
Aug 10, 2007·American Journal of Physiology. Cell Physiology·Andrés E Perez BayFernando D Marengo
Oct 18, 2012·Physiological Reviews·Haruo KasaiHiroshi Tokumaru
Nov 27, 2019·Acta Physiologica·José Moya-DíazFernando D Marengo
Sep 10, 2017·Pflügers Archiv : European journal of physiology·Madhurima DharaDieter Bruns

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