Estimation of postmortem interval by vitreous potassium evaluation with a novel fluorescence aptasensor

Scientific Reports
Yanjun DingJifeng Cai

Abstract

Estimation of postmortem interval (PMI) is a central role in medico-legal identification. Analysis of vitreous potassium ions (K+) concentration is frequently used by forensic workers to estimate PMI. This paper describes interdisciplinary research to introduce fluorescence sensing techniques into forensic medicine. On the basis of silver nanoclusters (AgNCs) probe stabilized by DNA, a simple and highly sensitive fluorescence aptasensor has been proposed to selectively detect K+ ions. The linear range for K+ ions was found to be 0.1 nM-1 mM, with limit of detection of 0.06 nM. Moreover, 63 vitreous humour cases within 36 h after death were further studied to verify the utility of K+ ions in estimating the PMI. By the fluorescence aptasensor method, a new formula was built to determine the postmortem interval based on K+ ions concentration: PMI(h) = -0.55 + 1.66 × CK+(r = 0.791). And the real significance of this research was demonstrated by additional 6 cases with known PMIs. In comparison with the conventional method, the presented aptasensor strategy is cost-effective and easy in measuring vitreous K+, which may be potentially a better way for estimation of PMI in medico-legal practice.

References

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Citations

Oct 17, 2017·Analytical and Bioanalytical Chemistry·Yanjun DingLagabaiyla Zha
May 10, 2019·Metabolomics : Official Journal of the Metabolomic Society·Emanuela LocciErnesto d'Aloja
Jun 12, 2020·Science Advances·Leah S WilkMaurice C G Aalders
Nov 5, 2019·Critical Reviews in Clinical Laboratory Sciences·Maryam HeydariMajid Rezayi
Sep 17, 2020·Science Advances·Leah S WilkMaurice C G Aalders

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Methods Mentioned

BETA
Fluorescence

Software Mentioned

SPSS

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