Estimation of size of clonal unit for keratinocytes in normal human skin.

Archives of Pathology & Laboratory Medicine
Vijaya ChaturvediBrian J Nickoloff

Abstract

It has been suggested that keratinocyte (KC) stem cells reside at the epicenter of a clonal population of cells. To estimate the territory or surface area covered by a single stem-cell-derived KC population in human skin, clonal skin maps were created from 3 healthy adult women and from normal skin of a psoriatic patient. Two hundred fifty-eight punch biopsy samples of various sizes (ranging from 2 to 8 mm in diameter) were analyzed for clonality employing X chromosome inactivation patterns at the human androgen receptor gene (HUMARA) locus. DNA was isolated and clonality established by significant decrease of either maternal or paternal X chromosome band patterns following restriction enzyme digestion, polymerase chain reaction amplification, and gel electrophoresis. Fifty-three (41%) of 128 two-mm biopsies were clonal, whereas only 6 (14%) of 43 three-mm, 5 (14%) of 36 four-mm, and 3 (8%) of 35 five-mm biopsies revealed a clonal population of KCs. By contrast, in 5 different biopsies from a psoriatic patient, including 4- or 5-mm sizes, all but 1 were clonal; even an 8-mm biopsy contained a clonal population of KCs. Mantel-Haenszel chi(2) analysis revealed a P value of.001, reflecting a strong trend in probability for presenc...Continue Reading

References

Jan 1, 1985·Cell and Tissue Research·I C Mackenzie, J R Bickenbach
May 1, 1986·The Histochemical Journal·B A PonderM M Wilkinson
Jan 1, 1985·Journal of the American Academy of Dermatology·E M FarberA Strefling
Jan 1, 1972·Biological Reviews of the Cambridge Philosophical Society·M F Lyon
Jun 1, 1981·The Journal of Investigative Dermatology·I C MackenzieL Peterson
Nov 24, 1994·The New England Journal of Medicine·A S PallerE Fuchs
Jan 13, 1995·Cell·P H JonesF M Watt
Mar 1, 1996·The Journal of Investigative Dermatology·D S WalshM Peacocke
Apr 3, 1997·The New England Journal of Medicine·C S RabkinZ Zhuang
Jan 7, 1998·Nature Biotechnology·H DengP A Khavari
Feb 3, 1998·Journal of the American Academy of Dermatology·D S WalshH C Tsou
Oct 15, 1998·Experimental Cell Research·J R Bickenbach, E Chism
Jun 9, 1999·Proceedings of the National Academy of Sciences of the United States of America·A J ZhuF M Watt
Feb 13, 2001·Experimental Dermatology·M DunnwaldJ R Bickenbach

❮ Previous
Next ❯

Citations

Nov 17, 2010·Proceedings of the National Academy of Sciences of the United States of America·Christian HafnerFrancisco X Real
Jan 29, 2005·The Journal of Investigative Dermatology·Soosan Ghazizadeh, Lorne B Taichman
Jun 16, 2005·The Journal of Investigative Dermatology·Brian J NickoloffEli Pikarsky
Apr 24, 2008·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Mariko OkadaYoshinobu Sugino
Jun 25, 2008·Stem Cell Reviews·Lauren R Strachan, Ruby Ghadially
Jun 11, 2010·Integrative Biology : Quantitative Biosciences From Nano to Macro·Martin RuetzeAnja Knott
Jun 18, 2004·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Boudewijn J M BraakhuisRuud H Brakenhoff
Aug 25, 2011·Journal of Dermatological Science·Martin RuetzeAnja Knott

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.