PMID: 11605987Oct 19, 2001Paper

Estimation of the abundance of the cadmium resistance gene cadA in microbial communities in polluted estuary water

Research in Microbiology
Cécile OgerFabienne Petit

Abstract

We describe herein a molecular method for estimating the abundance of the cadA gene, which encodes a Cd2+/ATPase protein transporter, in bacterial DNA extracted from samples of environmental water. Competitive polymerase chain reaction (cPCR) may be the most appropriate technique for assessing the prevalence of the cadA gene in microbial communities in highly heterogeneous and polluted environments, such as the Seine estuary (France). We describe the development of this method: (i) the choice of two specific primers, based on the sequences encoding the cadmium binding site and the ion channel domains; (ii) the construction of a competitor sequence and assessment of its amplification efficiency; and (iii) the estimation of the copy number of the cadA gene. The cadA content in the bacterial community is expressed as the number of gene copies per ng of total DNA extracted, which is independent of the DNA extraction yield. This molecular procedure was improved to analyze cadA levels in bacterial DNA extracted from estuary water accidentally contaminated with cadmium. Results revealed a subsequent increase in the copy number of the cadA gene in the microbial community.

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Citations

Jun 5, 2014·Environmental Science and Pollution Research International·Assia KaciThierry Berthe
Jun 10, 2015·The Science of the Total Environment·Cristina Becerra-CastroCélia M Manaia
Mar 13, 2016·Bulletin of Environmental Contamination and Toxicology·Bulent Icgen, Fadime Yilmaz
Nov 21, 2015·Environmental Science and Pollution Research International·Bulent Icgen, Fadime Yilmaz
Jun 28, 2003·FEMS Microbiology Reviews·Dayle K Blencowe, Andrew P Morby
May 3, 2015·Environmental Science and Pollution Research International·Assia KaciThierry Berthe
Dec 21, 2007·FEMS Microbiology Ecology·Anna LazzaroBeat Frey
Jan 19, 2018·Journal of Applied Microbiology·D BravoC Leon-Moreno
Mar 2, 2012·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Sangeeta ChoudharyPinaki Sar
Oct 26, 2005·Journal of Theoretical Biology·Fyodor A Kondrashov, Alexey S Kondrashov

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