PMID: 6401126Oct 1, 1984Paper

Estimation of the dermal carcinogenic activity of petroleum fractions using a modified Ames assay

Cell Biology and Toxicology
G R BlackburnC R Mackerer

Abstract

The Ames Salmonella/microsomal activation mutagenesis assay has been adapted to improve sensitivity to complex hydrocarbon mixtures produced by the refining of petroleum. Extraction of oil samples with dimethyl sulfoxide produces aqueous-compatible solutions that more easily interact with the tester bacteria. These extracts, therefore, produce higher revertant values than do equivalent volumes of oil delivered neat or dissolved in organic solvent. Parallel increases in the liver microsomal S-9 concentration further improve the sensitivity of the assay, allowing detection of mutagenicity in otherwise inactive samples. The effect of increased microsomal fraction from rodent liver is apparently attributable to the higher levels of activating enzymes rather than to the concomitant increase in the overall hydrophobicity of the test system. The modified assay has been used to rank thirteen petroleum-derived oils and a corn oil control for relative mutagenic activity. This ranking closely correlates (r = 0.97) with potency rankings of the same samples previously determined from dermal carcinogenicity bioassays.

Citations

Jan 1, 1991·International Archives of Occupational and Environmental Health·M Granella, E Clonfero
Dec 19, 2012·Regulatory Toxicology and Pharmacology : RTP·Thomas M GrayRichard H McKee
May 21, 2013·Regulatory Toxicology and Pharmacology : RTP·Richard H McKeeThomas M Gray
Jun 12, 2013·Regulatory Toxicology and Pharmacology : RTP·Randy N RothThomas M Gray
Feb 26, 1999·Mutation Research·R T PrzygodaJ J Freeman
Aug 1, 1990·Journal of Applied Toxicology : JAT·R H McKeeC Chauzy
Jan 1, 1994·Environmental and Molecular Mutagenesis·R H McKeeR T Przygoda
Dec 1, 1985·Regulatory Toxicology and Pharmacology : RTP·J T Barr
Aug 1, 1987·Journal of Applied Toxicology : JAT·A J Ingram, P Grasso
Jul 1, 1997·Journal of Toxicology and Environmental Health·M H FeustonC E Hamilton
Sep 1, 1997·Journal of Toxicology and Environmental Health·M H FeustonC R Mackerer
Jun 1, 1986·Environmental Research·J S DutcherR O McClellan
Dec 20, 2011·Regulatory Toxicology and Pharmacology : RTP·Peter J BoogaardGary D Minsavage
Sep 13, 2013·Regulatory Toxicology and Pharmacology : RTP·Charles R ClarkPatrick W Beatty
Sep 1, 2011·International Journal of Toxicology·Katy O GoyakJames J Freeman
Feb 26, 2014·International Journal of Toxicology·Walden E DalbeyRussell White
Nov 2, 2013·International Journal of Toxicology·Richard H McKeeKaty Olsavsky Goyak
Apr 1, 1990·Journal of Applied Toxicology : JAT·A J Ingram
Jan 16, 2020·Critical Reviews in Toxicology·Ralph PirowAndreas Luch
Jul 11, 2012·The Journal of Pharmacy and Pharmacology·Talib HussainChandana Venkateswara Rao
Nov 3, 2017·Critical Reviews in Toxicology·Anthony J KriechAnthony J Riley
Oct 5, 2011·Inhalation Toxicology·David TrumboreL Daniel Maxim
May 23, 2021·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Lenny KameliaPeter J Boogaard

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.