Estimation of the hydrogen peroxide producing capacities of liver and cardiac mitochondria isolated from C57BL/6N and C57BL/6J mice

Free Radical Biology & Medicine
Catherine OldfordRyan J Mailloux

Abstract

Here, we examined the hydrogen peroxide (H2O2) producing capacities of pyruvate dehydrogenase (PDH), α-ketoglutarate dehydrogenase (KGDH), proline dehydrogenase (PRODH), glycerol-3-phosphate dehydrogenase (G3PDH), succinate dehydrogenase (SDH; complex II), and branched-chain keto acid dehydrogenase (BCKDH), in cardiac and liver mitochondria isolated from C57BL/6N (6N) and C57BL/6J (6J) mice. Various inhibitor combinations were used to suppress ROS production by complexes I, II, and III and estimate the native rates of H2O2 production for these enzymes. Overall, liver mitochondria from 6N mice produced ∼2-fold more ROS than samples enriched from 6J mice. This was attributed, in part, to the higher levels of glutathione peroxidase-1 (GPX1) and catalase (CAT) in 6J mitochondria. Intriguingly, PDH, KGDH, and SDH comprised up to ∼95% of the ROS generating capacity of permeabilized 6N liver mitochondria, with PRODH, G3PDH, and BCKDH making minor contributions. By contrast, BCKDH accounted for ∼34% of the production in permeabilized 6J mitochondria with KGDH and PRODH accounting for ∼23% and ∼19%. G3PDH produced high amounts of ROS, accounting for ∼52% and ∼39% of the total H2O2 generating capacity in 6N and 6J heart mitochondria. PRO...Continue Reading

Citations

Jun 6, 2020·Antioxidants·Ryan J Mailloux
Aug 8, 2019·International Journal of Molecular Sciences·Simona DamianoMariarosaria Santillo
Jul 28, 2020·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Wing Yip Tam, Kwok-Kuen Cheung
Sep 24, 2020·Nutrients·Maria Michela CesareRossella Di Stefano
Sep 8, 2019·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Teodora Alexa-StratulatChiara Riganti
Jan 1, 2021·Free Radical Biology & Medicine·Chidozie N OkoyeCollins Kamunde
Feb 25, 2021·Chemical Communications : Chem Comm·Jiayu ZhangYi Hu
Apr 2, 2021·Antioxidants & Redox Signaling·Francesco Bellanti, Gianluigi Vendemiale
Jan 15, 2021·Experimental Animals·Kazuyuki Mekada, Atsushi Yoshiki
Aug 22, 2021·Antioxidants & Redox Signaling·Eftekhar Eftekharpour, Paul Fernyhough

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