PMID: 7219207Jan 1, 1980Paper

Estimation of type-specific neutralizing antibody to herpes simplex virus type 2 in uterine cervical cancer patients by a new absorption method

Microbiology and Immunology
T Kawana, K Yoshino

Abstract

A simple method of estimating type-specific neutralizing antibody to type 2 herpes simplex virus (HSV-2) was devised with the use of the microneutralization system. Serially diluted serum was mixed in the well with a constant amount of type 1 virus (HSV-1), and after 3 days' incubation at 37 C, the plate was irradiated with ultraviolet light. The absorbing HSV-1 consisted of culture fluid plus an extract of infected Vero cells not especially concentrated. The well then received indicator HSV-1 or HSV-2, and after being left at 37 C for 1 hr a suspension of dispersed Vero cells was dropped into the wells, following our standard neutralization procedure. Preliminary tests with rabbit antisera showed that even a low level of HSV-2 antibody was detected by this method, unless an exceptionally high titer of HSV-1 antibody originally coexisted with the HSV-2 antibody. Sera from acutely infected persons testified to the specificity of the antibody so detected. It was revealed by means of the new technique that the rate of HSV-2 antibody was significantly higher in uterine cervical cancer patients than in control women. There was no correlation between the clinical stage of cervical cancer and the presence of HSV-2 antibody.

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Citations

Sep 1, 1986·Journal of Virological Methods·F K LeeA Nahmias
Jan 1, 1982·Microbiology and Immunology·K YoshinoK Abe
Mar 25, 2008·Journal of Medical Virology·Hisatoshi KanekoTatsuo Suzutani
Jan 1, 1988·Acta Oto-laryngologica. Supplementum·J KoideT Kurata

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