Estimation of uptake of humic substances from different sources by Escherichia coli cells under optimum and salt stress conditions by use of tritium-labeled humic materials.

Applied and Environmental Microbiology
Natalia A KulikovaEugenia A Tsvetkova

Abstract

The primary goal of this paper is to demonstrate potential strengths of the use of tritium-labeled humic substances (HS) to quantify their interaction with living cells under various conditions. A novel approach was taken to study the interaction between a model microorganism and the labeled humic material. The bacterium Escherichia coli was used as a model microorganism. Salt stress was used to study interactions of HS with living cells under nonoptimum conditions. Six tritium-labeled samples of HS originating from coal, peat, and soil were examined. To quantify their interaction with E. coli cells, bioconcentration factors (BCF) were calculated and the amount of HS that penetrated into the cell interior was determined, and the liquid scintillation counting technique was used as well. The BCF values under optimum conditions varied from 0.9 to 13.1 liters kg(-1) of cell biomass, whereas under salt stress conditions the range of corresponding values increased substantially and accounted for 0.2 to 130 liters kg(-1). The measured amounts of HS that penetrated into the cells were 23 to 167 mg and 25 to 465 mg HS per kg of cell biomass under optimum and salt stress conditions, respectively. This finding indicated increased penetrat...Continue Reading

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Citations

Oct 13, 2015·New Biotechnology·Karolína PádrováVladimír Jirků
Sep 7, 2020·Environmental Pollution·Dechao DuanJiyan Shi
May 20, 2018·Biochimica Et Biophysica Acta. Bioenergetics·Joana M DantasCarlos A Salgueiro
Jun 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Natalia A Kulikova, Irina V Perminova
Nov 6, 2018·Journal of Agricultural and Food Chemistry·Qiao XuJiyan Shi

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