PMID: 841622Feb 1, 1977Paper

Estradiol-17beta receptors in the immature rat ovary

Steroids
S Saiduddin, H P Zassenhaus

Abstract

Estradiol binding components in the cytosol and nuclear fractions of the ovary from immature rats (22-28 days old) were characterized by in vitro methods. Several of the biochemical characteristics of the estradiol binding components in the ovarian tissue were compared with the estradiol receptor from the uterus. The results suggest that the ovarian estradiol binding components are similar to the specific high affinity estradiol receptors in the uterus. In the cytosol of intact rat ovary a significant fraction of the total binding sites was found to be occupied, presumably by the endogenous estrogen. Following hypophysectomy there was a significant increase in the available cytosol binding sites. Evidence for translocation of cytosol receptor-estrogen (RE) complex to the nucleus was obtained for the ovary. The sedimentation properties of the RE complex of the ovary and the uterus are similar. The ovarian cytosol RE complex sediments at 7-8S in glycerol gradients at low ionic strength and at 4S in sucrose gradients at high ionic strength. Following extraction with 0.4 M KCl the ovarain nuclear RE complex sediments at 5S in sucrose gradients which is identical to that of the uterine nuclear receptor.

References

Mar 1, 1974·Journal of Reproduction and Fertility·R G Edwards
Apr 1, 1972·Journal of Steroid Biochemistry·E V JensenE R DeSombre
Feb 15, 1974·Science·B W O'Malley, A R Means
Jun 1, 1974·Proceedings of the Society for Experimental Biology and Medicine·S Saiduddin, G E Milo
Oct 9, 1973·Biochemistry·J A KatzenellenbogenH N Myers
Dec 1, 1972·Endocrinology·E O ReiterG T Ross
Nov 1, 1971·Proceedings of the Society for Experimental Biology and Medicine·S Saiduddin
Jan 1, 1968·The Journal of Clinical Endocrinology and Metabolism·S G Korenman

❮ Previous
Next ❯

Citations

Jun 28, 2003·FEBS Letters·Zhang WeihuaJan-Ake Gustafsson
Jun 16, 1999·Endocrine Reviews·J F Couse, K S Korach
Jun 16, 2001·Endocrine Reviews·S F PalterE Y Adashi
May 26, 1999·Endocrinology·S L FitzpatrickD E Frail
Feb 1, 1997·Molecular Endocrinology·M ByersO K Park-Sarge
Jan 1, 1983·Journal of Receptor Research·S Saiduddin, H P Zassenhaus
Dec 1, 1982·Asia-Oceania Journal of Obstetrics and Gynaecology·H KusanishiH Okada
Jun 1, 1982·Asia-Oceania Journal of Obstetrics and Gynaecology·T TamayaH Okada
Apr 1, 1982·General and Comparative Endocrinology·C E Young, I R McDonald

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
S SajiJ A Gustafsson
Proceedings of the National Academy of Sciences of the United States of America
Z WeihuaJ A Gustafsson
Proceedings of the National Academy of Sciences of the United States of America
G G KuiperJ A Gustafsson
© 2021 Meta ULC. All rights reserved