Estradiol and testosterone modulate the tissue-specific expression of ghrelin, ghs-r, goat and nucb2 in goldfish

General and Comparative Endocrinology
Juan I BertucciSuraj Unniappan

Abstract

Ghrelin, and nesfatin-1 (encoded by nucleobindin2/nucb2) are two metabolic peptides with multiple biological effects in vertebrates. While sex steroids are known to regulate endogenous ghrelin and NUCB2 in mammals, such actions by steroids in fish remain unknown. This study aimed to determine whether estradiol (E2) and testosterone (T) affects the expression of preproghrelin, ghrelin/growth hormone secretagogue receptor (GHS-R), ghrelin O-acyl transferase (GOAT) and NUCB2 in goldfish (Carassius auratus). First, a dose-response assay was performed in which fish were intraperitoneally (ip) implanted with pellets containing 25, 50 or 100 μg/g body weight (BW) of E2 or T. It was found that sex steroids (100 μg/g BW) administered for 2.5 days achieved the highest E2 or T in circulation. In a second experiment, fish were ip implanted with pellets containing 100 μg/g BW of E2, T or without hormone (control). RT-qPCR analyses at 2.5 days post-administration show that gut preproghrelin and GOAT expression was upregulated by both E2 and T treatments, while the same effect was observed for GHS-R only in the pituitary. Both treatments also reduced hypothalamic preproghrelin mRNA expression. NUCB2 expression was increased in the forebrain o...Continue Reading

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Citations

Sep 3, 2016·Current Opinion in Pharmacology·Philip Prinz, Andreas Stengel
Nov 1, 2018·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Juan Ignacio BertucciLuis Fabián Canosa
Aug 15, 2018·Frontiers in Endocrinology·Miquel Perelló-AmorósJoaquim Gutiérrez
Dec 25, 2019·International Journal of Endocrinology·Mahmoud Mustafa Ali AbulmeatySuhail Razak
May 30, 2019·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Alicia T PateGina L C Yosten
Jun 6, 2019·Theriogenology·Azadeh Hatef, Suraj Unniappan
Aug 1, 2021·General and Comparative Endocrinology·Krittika DotaniaUmesh Rai

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