Estradiol enhances thiazide-sensitive NaCl cotransporter density in the apical plasma membrane of the distal convoluted tubule in ovariectomized rats

The Journal of Clinical Investigation
J W VerlanderS C Hebert

Abstract

Recent data suggest that sex hormones affect the thiazide-sensitive NaCl cotransporter (TSC) density or binding capacity (Chen, Z., D.A. Vaughn, and D.D. Fanestil. 1994. J. Am. Soc. Nephrol. 5:1112-1119). Thus, we determined the effect of ovariectomy (OVX) and estrogen replacement on the ultrastructural localization of TSC in rat kidney using immunocytochemistry. Kidneys of intact female (CON) and OVX rats fed ad libitum for 6 and 9 wk or pair-fed for 9 wk were processed for transmission electron microscopy. Immunogold localization of rat TSC (rTSC1) demonstrated intense label in the apical plasma membrane of CON distal convoluted tubule (DCT). In OVX DCT, rTSC1 label and apical plasma membrane microprojections were decreased. Western blots of renal membrane protein from pair-fed CON and OVX revealed bands at 129-135 kD, but the OVX signal was reduced. Morphometric analyses demonstrated that injecting 10 microg/ kg body weight 17beta-estradiol subcutaneously 4x/wk in OVX rats restored DCT apical microprojections and label density for rTSC1. Thus, in OVX rats (a) rTSC1 immunoreactive renal membrane protein is reduced; (b) apical plasma membrane complexity and immunogold label for rTSC1 in DCT is decreased; and (c) estradiol repl...Continue Reading

References

Aug 1, 1992·The Journal of Clinical Investigation·F A Gesek, P A Friedman
Dec 1, 1992·The Journal of Clinical Investigation·T Naveh-ManyJ Silver
Jan 1, 1992·The Journal of Steroid Biochemistry and Molecular Biology·C B WhorwoodP M Stewart
Nov 1, 1974·Kidney International·N P Christy, J C Shaver
Dec 1, 1974·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·I W McLean, P K Nakane
Apr 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·G GambaS C Hebert
Jul 1, 1996·Kidney International·M D PlotkinS C Hebert

❮ Previous
Next ❯

Citations

Oct 25, 2006·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Eva Riveira-MunozOlivier Devuyst
May 5, 2006·Current Hypertension Reports·Carolyn A Ecelbarger, Swasti Tiwari
Oct 3, 1999·Kidney International·G Gamba
Dec 28, 1999·Kidney International·E Grossman, F H Messerli
Feb 13, 2001·Kidney International·D N CruzUNKNOWN Yale Gitelman's and Bartter's Syndrome Collaborative Study Group
Jan 19, 2013·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Miriam R BerryFiona E Karet Frankl
Aug 10, 2001·Current Opinion in Nephrology and Hypertension·M A Knepper, H L Brooks
Jun 22, 2013·Pharmacogenetics and Genomics·Caroline F ThornTeri E Klein
Jan 28, 2005·Kidney International·Franz H Messerli
Jul 3, 2009·American Journal of Physiology. Renal Physiology·Volker VallonShinichi Uchida
Nov 27, 2004·American Journal of Nephrology·Gheun-Ho Kim
Dec 7, 2013·Pflügers Archiv : European journal of physiology·Arthur D MoesEwout J Hoorn
Oct 15, 2011·Cellular and Molecular Life Sciences : CMLS·C Esteva-FontP Fernández-Llama
Jun 7, 2012·Climacteric : the Journal of the International Menopause Society·N J HeoJ S Han
Aug 19, 2003·The Journal of Physiology·Nina S StachenfeldDavid L Keefe
Jun 29, 2010·The American Journal of the Medical Sciences·Bushra AbbasiLaurie R Sprabery
Jan 31, 2016·Chinese Journal of Integrative Medicine·Jie-Wei LuoWei-Hua Li
Apr 23, 2013·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·Eline M RodenburgLoes E Visser
Oct 3, 2014·Endocrine Journal·Jiewei LuoWeihua Li
Apr 27, 2012·British Journal of Clinical Pharmacology·Eline M RodenburgLoes E Visser
Jul 26, 2011·The American Journal of Cardiology·Norela V OcampoRamdas G Pai
Apr 7, 2015·Acta Biochimica Et Biophysica Sinica·Linghong WangXiulan Su
Oct 27, 2016·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·Y ZhangN Tong
Jul 6, 2013·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·J B GraceliM R Moysés
Mar 22, 2006·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Richard A ColemanJames B Wade
Jun 14, 2018·American Journal of Physiology. Renal Physiology·Erin WolfMatthew A Sparks
Aug 5, 2017·Journal of the American Society of Nephrology : JASN·Luciana C VeirasAlicia A McDonough
Mar 3, 2007·Journal of the American Society of Nephrology : JASN·Eva Riveira-MunozUNKNOWN Belgian Network for Study of Gitelman Syndrome
Sep 8, 2005·American Journal of Physiology. Renal Physiology·Young-Hee KimSusan M Wall
Oct 26, 2001·Drug Safety : an International Journal of Medical Toxicology and Drug Experience·D C Felmeden, G Y Lip
Apr 27, 2007·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Feng LiMaureen Keller-Wood
Jan 15, 2015·American Journal of Physiology. Renal Physiology·Lorena Rojas-VegaGerardo Gamba
May 18, 2007·American Journal of Physiology. Renal Physiology·Monica B SandbergArvid B Maunsbach

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.