Estradiol inhibits adhesion and promotes apoptosis in murine osteoclasts in vitro

The Journal of Steroid Biochemistry and Molecular Biology
D SaintierM E Cohen-Solal

Abstract

Osteoporosis caused by estrogen deficiency is characterized by enhanced bone resorption mediated by osteoclasts. Adhesion to bone matrix and survival of differentiated osteoclasts is necessary to resorb bone. The aim of our study was to investigate the in vitro effects of estradiol on murine osteoclasts. RAW 264.7 cells treated with 30 ng/ml RANK-L were used as a model for osteoclastogenesis. Estradiol (10(-8)M) for 5 days induced an inhibition of osteoclast differentiation and beta3 expression. Estradiol inhibited significantly the adhesion of mature osteoclasts by 30%. Furthermore estradiol-induced apoptosis shown by with nuclear condensation and Bax/Bcl2 ratio. In addition, estradiol enhanced caspase-3, -8 and -9 activities. This effect completely disappeared using specific caspase-8 inhibitor. However, increased caspase-3 activity by estradiol was observed in the presence of caspase-9 inhibitor, indicating the preferential involvement of caspase-8 pathway. Fas and FasL mRNA expression was not regulated by estradiol. However, estradiol enhanced caspase-3 activity in Fas-induced apoptosis on mature osteoclasts, suggesting that this might interact with the Fas-signaling pathway. These data suggest that estradiol decreases bone...Continue Reading

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Citations

Jun 13, 2009·Breast Cancer Research : BCR·Joan S Lewis-Wambi, V Craig Jordan
Feb 23, 2012·Journal of Molecular Endocrinology·Jerzy-Roch Nofer
Nov 13, 2012·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Francesca RossiSabatino Maione
Sep 29, 2011·Chemico-biological Interactions·Leilei BaoQiaoyan Zhang
Jul 21, 2011·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Hong ChenJohn Adams
Aug 5, 2011·Journal of Cellular Biochemistry·Jun Wang, Paula H Stern
Nov 19, 2011·Journal of Anatomy·Ana Paula de Souza FaloniPaulo Sérgio Cerri
Nov 6, 2013·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·John R HawseMalayannan Subramaniam
Jun 30, 2015·BioMed Research International·Qi XiongWei Ge
Jan 13, 2015·Journal of Endodontics·João Eduardo Gomes-FilhoLuciano Tavares Angelo Cintra
Apr 5, 2007·Journal of Cellular Biochemistry·Paula H Stern
Nov 4, 2016·Physiological Reviews·Maria AlmeidaStavros C Manolagas
Jun 20, 2019·Journal of Molecular Histology·Shinji HiyamaRyuji Terayama
Jan 7, 2015·The Journal of Biological Chemistry·Yong Deok LeeHong-Hee Kim
Aug 7, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Manikandan Subramanian, Chandrima Shaha

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