Estradiol inhibits NLRP3 inflammasome in fibroblast-like synoviocytes activated by lipopolysaccharide and adenosine triphosphate

International Journal of Rheumatic Diseases
Jianfeng ShiBo Shen

Abstract

Nucleotide binding domain and leucine-rich repeat pyrin 3 domain (NLRP3) inflammasome is known for activating pro-inflammatory cytokines in knee osteoarthritis (OA). This study was performed to identify whether NLRP3 inflammasome can be triggered by lipopolysaccharides (LPS) and adenosine triphos adenine (ATP), which are positively related with knee OA severity in joint-spaces, in human fibroblast-like synoviocytes (FLS), and to identify whether estrogen would inhibit the activation of NLRP3 inflammasome. In the present study, human FLS were isolated from the knee OA in patients during arthroplasty, and were treated with LPS and ATP in the presence or absence of estradiol (E2). The messenger RNA (mRNA) and protein levels of NLRP3 components were analyzed by real-time polymerase chain reaction and western blotting, respectively. Enzyme-linked immunosorbent assay (ELISA) was used to examine interleukin (IL)-1β and IL-18 content in the supernatant. Estrogen receptor α inhibitor MPP and β inhibitor PHTPP were used to explore how E2 works. Our results demonstrated that treatment with LPS and ATP increased significantly both in mRNA and protein levels of all the NLRP3 inflammasome components, and triggered the NLRP3 inflammasome, fol...Continue Reading

References

Nov 24, 1999·Osteoarthritis and Cartilage·T UshiyamaS Hukuda
Nov 6, 2004·Arthritis and Rheumatism·Laura D CarboneUNKNOWN Health, Aging and Body Composition Study
Feb 26, 2005·Annals of the Rheumatic Diseases·M J BenitoB Bresnihan
Sep 23, 2006·Journal of the Society for Gynecologic Investigation·Wolf DietrichWalter Tschugguel
Oct 15, 2009·Immunology·Laeticia KollyAlexander So
Aug 19, 2011·Cellular & Molecular Biology Letters·Jia Gu HuangBing Zhang
Aug 23, 2011·Proceedings of the National Academy of Sciences of the United States of America·Chengcheng JinRichard A Flavell
Dec 8, 2011·World Journal of Gastroenterology : WJG·Michal GanzGyongyi Szabo
Jul 25, 2012·International Journal of Molecular Medicine·Zhaozong FuJianming Jiang
Jan 29, 2013·Joint, Bone, Spine : Revue Du Rhumatisme·Marta Martín-Millán, Santos Castañeda
May 25, 2013·Nature Reviews. Immunology·Eicke LatzAndrea Stutz
Jul 22, 2014·Nature·Lieselotte Vande WalleMohamed Lamkanfi
Jan 1, 2014·Journal of Long-term Effects of Medical Implants·Yuya TakakuboMichiaki Takagi
May 26, 2015·Laboratory Investigation; a Journal of Technical Methods and Pathology·Qing WeiLihui Han
Aug 21, 2015·Annals of the Rheumatic Diseases·Mary ConnollyUrsula Fearon
Dec 15, 2015·Scientific Reports·Shui-Xing YuYong-Jun Yang
May 20, 2016·Nature Reviews. Rheumatology·Richard F LoeserBrian O Diekman
Nov 16, 2016·Oxidative Medicine and Cellular Longevity·Roshni ThakkarDarrell Brann
Nov 16, 2016·Neuroimmunomodulation·Yunnan LuWeifeng Luo
Apr 7, 2017·Nature Reviews. Rheumatology·Ali MobasheriUrsula Fearon

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Citations

Nov 14, 2018·Clinical and Experimental Immunology·M H J van den Bosch
Sep 13, 2019·Journal of Orthopaedic Surgery and Research·Yan ZuHong-Juan Yan
Feb 3, 2018·Molecular Medicine Reports·Ling-Rui ZhaoLi Zhang
Dec 2, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Lina Y El-SharkawySally Freeman
Mar 12, 2021·The Journal of Nutritional Biochemistry·Mi Nam LeeMunkyong Pae
Jul 13, 2021·Frontiers in Endocrinology·Yi-Shang YanHe-Feng Huang

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