Estradiol negatively regulates HIV-LTR promoter activity in glial cells

AIDS Research and Human Retroviruses
Melinda E WilsonA Nath

Abstract

HIV-associated dementia results from neuronal loss and an alteration of neuronal function due to a loss of synapses. While HIV infection in astrocytes is limited, astrocytes exhibit a chronic nonproductive infection that can lead to the release of neurotoxic proteins. Additionally, infection can disrupt the normal neurotrophic role of astrocytes that results in neuronal death. Gonadal steroid hormones are known to act as trophic and protective factors in the brain under a variety of normal and pathological conditions. In the present study, to determine if estrogen plays a role in the ability of Tat to function as a transcriptional activator within astrocytes, we examined the effect of estrogen on regulation of viral transcription. We utilized an immortalized human astrocyte cell line (SVGA) stably transfected with a reporter plasmid containing the HIV-1IIIB LTR driving the chloramphenicol acetyltransferase (CAT) gene. The amount of transcriptional activity was measured by quantifying the amount of CAT produced. We determined that 17beta-estradiol treatment (1 nM) had no effect on basal LTR activity. Following transfection with a Tat-expressing plasmid, there was a 100-fold increase in CAT production. This induction was reduced ...Continue Reading

References

Nov 1, 1992·Journal of Neuropathology and Experimental Neurology·E MasliahC A Wiley
Jun 23, 1993·Annals of the New York Academy of Sciences·M Lotz, P Seth
Aug 15, 1997·Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology : Official Publication of the International Retrovirology Association·R A Clark, R Bessinger
Sep 2, 1998·British Journal of Clinical Pharmacology·D OuelletG R Granneman
Nov 11, 1998·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·D B DubalP M Wise
Jan 19, 2000·Nucleic Acids Research·L A PereiraN J Deacon
Jan 6, 2001·Frontiers in Neuroendocrinology·P M WiseY Liu
Feb 7, 2001·The Journal of Clinical Endocrinology and Metabolism·S M HarmanUNKNOWN Baltimore Longitudinal Study of Aging
Mar 27, 2001·Annual Review of Pharmacology and Toxicology·S J Lee, B S McEwen
May 29, 2002·Proceedings of the National Academy of Sciences of the United States of America·Srikanth YellayiPaul S Cooke
Apr 18, 2003·Endocrine Reviews·Barbara B Sherwin
May 25, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Gwenn A GardenRichard S Morrison
Jun 24, 2004·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Kevin R RobertsonColin D Hall
Dec 8, 2004·Journal of Neuroimmunology·Justin C McArthur

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Citations

May 3, 2007·AIDS Research and Therapy·Melinda E WilsonAnthony J Bisotti
Apr 25, 2013·PloS One·Marta Rodriguez-GarciaCharles R Wira
Aug 23, 2008·Journal of Basic Microbiology·Zhe MaWentao Qiao
Oct 15, 2014·AIDS Research and Human Retroviruses·Rowena E Johnston, Mary M Heitzeg
Feb 24, 2016·Journal of the International AIDS Society·Sara GianellaCatherine Godfrey
Oct 16, 2021·Journal of Neuroendocrinology·Mohammed F SalahuddinJason J Paris

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