Estradiol triggers sonic-hedgehog-induced angiogenesis during peripheral nerve regeneration by downregulating hedgehog-interacting protein.

Laboratory Investigation; a Journal of Technical Methods and Pathology
Haruki SekiguchiDouglas W Losordo

Abstract

Both estradiol (E2) and Sonic Hedgehog (Shh) contribute to angiogenesis and nerve regeneration. Here, we investigated whether E2 improves the recovery of injured nerves by downregulating the Shh inhibitor hedgehog-interacting protein (HIP) and increasing Shh-induced angiogenesis. Mice were treated with local injections of E2 or placebo one week before nerve-crush injury; 28 days after injury, nerve conduction velocity, exercise duration, and vascularity were significantly greater in E2-treated mice than in placebo-treated mice. E2 treatment was also associated with higher mRNA levels of Shh, the Shh receptor Patched-1, and the Shh transcriptional target Gli1, but with lower levels of HIP. The E2-induced enhancement of nerve vascularity was abolished by the Shh inhibitor cyclopamine, and the effect of E2 treatment on Shh, Gli1, and HIP mRNA expression was abolished by the E2 inhibitor ICI. Gli-luciferase activity in human umbilical-vein endothelial cells (HUVECs) increased more after treatment with E2 and Shh than after treatment with E2 alone, and E2 treatment reduced HIP expression in HUVECs and Schwann cells without altering Shh expression. Collectively, these findings suggest that E2 improves nerve recovery, at least in part...Continue Reading

References

Sep 12, 1991·The New England Journal of Medicine·M J StampferC H Hennekens
Oct 30, 1980·Nature·C Nüsslein-Volhard, E Wieschaus
Jul 9, 2002·Brain Research·Rustem R IslamovAlexander K Murashov
Jul 19, 2002·JAMA : the Journal of the American Medical Association·Jacques E RossouwUNKNOWN Writing Group for the Women's Health Initiative Investigators
Aug 23, 2002·Brain Research. Molecular Brain Research·Melinda E WilsonPhyllis M Wise
Apr 15, 2003·Toxicology and Applied Pharmacology·Tsung-Ming ShihJohn H McDonough
Aug 15, 2003·Development·Hiroshi KawahiraMatthias Hebrok
Mar 17, 2004·Molecular and Cellular Neurosciences·J CoulombeM Ruat
Sep 11, 2004·Arteriosclerosis, Thrombosis, and Vascular Biology·Kengo F KusanoDouglas W Losordo
Nov 19, 2005·The Journal of Endocrinology·Niklas AnderssonClaes Ohlsson
Feb 16, 2006·Archives of Internal Medicine·Judith HsiaUNKNOWN Women's Health Initiative Investigators
May 12, 2006·Journal of General Internal Medicine·Shelley R SalpeterEdwin E Salpeter
Oct 27, 2009·The American Journal of Medicine·Shelley R SalpeterEdwin E Salpeter

❮ Previous
Next ❯

Citations

Dec 4, 2020·International Journal of Molecular Sciences·Nathan Moreau, Yves Boucher
Oct 24, 2013·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Bo HaiFei Liu
May 18, 2021·Frontiers in Physiology·Chun YaoBin Yu

❮ Previous
Next ❯

Methods Mentioned

BETA
hormone replacement therapy

Software Mentioned

StatViewTM

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.