Estradiol up-regulates estrogen receptor and progesterone receptor gene expression in specific ovine uterine cells

Biology of Reproduction
N H Ing, M B Tornesi

Abstract

The regulation of estrogen receptor (ER) and progesterone receptor (PR) genes is critical to estrogen and progesterone responsiveness of the uterus during the estrous cycle. A low dose of estradiol, given to ovariectomized ewes to mimic the preovulatory estrogen surge, acutely enhanced ER and PR gene expression in most uterine cells. Estradiol effects were measured at 12, 24, and 48 h post-injection (n = 6 ewes per time) with immunohistochemistry and in situ hybridization. Whereas vehicle-treated ovariectomized ewes demonstrated low to moderate ER and PR mRNA and protein expression, estradiol enhanced PR mRNA and protein expression (at 12 h and 24 h, respectively) more rapidly than ER mRNA and protein expression (at 24 h and 48 h, respectively) in most uterine cells. However, the timing and extent of the estradiol response depended partly upon cell type (epithelial, stromal, or myometrial), cell region (luminal, superficial, middle, or deep endometrial or myometrial), adjacent cells, and prior progesterone treatment. For example, PR mRNA up-regulation was prolonged in middle and deep endometrial stroma, but increases in PR protein expression were highest in superficial and middle endometrial compartments, including the luminal ...Continue Reading

Citations

Mar 20, 2012·Cell and Tissue Research·Ky G PohlerMichael F Smith
Nov 17, 2009·In Vitro Cellular & Developmental Biology. Animal·Andy Michael LaughlinNancy H Ing
Mar 19, 2004·General and Comparative Endocrinology·Noemí Custodia-LoraIan P Callard
Jul 18, 2001·The Journal of Steroid Biochemistry and Molecular Biology·J A RobertsonN H Ing
May 7, 2003·The Journal of Steroid Biochemistry and Molecular Biology·C PunyadeeraP Groothuis
May 28, 2003·The Journal of Steroid Biochemistry and Molecular Biology·Yuhua Z Farnell, Nancy H Ing
May 28, 2003·The Journal of Steroid Biochemistry and Molecular Biology·Yuhua Z Farnell, Nancy H Ing
Jan 12, 1999·The Journal of Steroid Biochemistry and Molecular Biology·J A RobertsonN H Ing
Oct 22, 2011·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·S DivariB Biolatti
Mar 7, 2009·Biology of Reproduction·Reno R AraujoMilo C Wiltbank
Jul 25, 2009·Acta Veterinaria Scandinavica·Ylva Hedberg AlmAnne-Marie Dalin
Jun 2, 2011·Journal of Molecular Endocrinology·M Bertan YilmazSerdar E Bulun
Apr 21, 2010·Reproduction : the Official Journal of the Society for the Study of Fertility·L A OkumuP Lonergan
Mar 8, 2005·Reproduction : the Official Journal of the Society for the Study of Fertility·S SukjumlongE Persson
Jan 25, 2014·Journal of Endocrinological Investigation·L VignozziM Maggi
Feb 22, 2012·The Journal of Steroid Biochemistry and Molecular Biology·Rainer W FürstSusanne E Ulbrich
Jul 14, 2010·The Journal of Steroid Biochemistry and Molecular Biology·Nancy H Ing
May 20, 2009·Frontiers in Neuroendocrinology·Colin J SaldanhaBradley J Walters
Feb 13, 2016·Hormone Molecular Biology and Clinical Investigation·Radhika Nagamangalam ShridharanAddicam Jagannadha Rao
Jan 12, 2007·Reproduction in Domestic Animals = Zuchthygiene·C KohlerB Hoffmann
Nov 12, 2013·International Journal of Experimental Pathology·Ricardo A FochiSebastião R Taboga
Aug 1, 2015·Pigment Cell & Melanoma Research·Ai-Young Lee
Aug 26, 2015·Reproduction in Domestic Animals = Zuchthygiene·S E Palma-VeraR Einspanier
Apr 22, 2006·Theriogenology·Linda Munson
Sep 13, 2005·Domestic Animal Endocrinology·Horacio Cárdenas, William F Pope
Jan 4, 2006·American Journal of Obstetrics and Gynecology·Eleanor M BottWendy J McLaren

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.