Estramustine Phosphate Inhibits TGF-β -Induced Mouse Macrophage Migration and Urokinase-Type Plasminogen Activator Production

Analytical Cellular Pathology (Amsterdam)
Sonja S MojsilovićJuan F Santibanez

Abstract

Transforming growth factor-beta (TGF-β) has been demonstrated as a key regulator of immune responses including monocyte/macrophage functions. TGF-β regulates macrophage cell migration and polarization, as well as it is shown to modulate macrophage urokinase-type plasminogen activator (uPA) production, which also contributes to macrophage chemotaxis and migration toward damaged or inflamed tissues. Microtubule (MT) cytoskeleton dynamic plays a key role during the cell motility, and any interference on the MT network profoundly affects cell migration. In this study, by using estramustine phosphate (EP), which modifies MT stability, we analysed whether tubulin cytoskeleton contributes to TGF-β-induced macrophage cell migration and uPA expression. We found out that, in the murine macrophage cell line RAW 264.7, EP at noncytotoxic concentrations inhibited cell migration and uPA expression induced by TGF-β. Moreover, EP greatly reduced the capacity of TGF-β to trigger the phosphorylation and activation of its downstream Smad3 effector. Furthermore, Smad3 activation seems to be critical for the increased cell motility. Thus, our data suggest that EP, by interfering with MT dynamics, inhibits TGF-β-induced RAW 264.7 cell migration para...Continue Reading

References

Oct 6, 1997·Proceedings of the National Academy of Sciences of the United States of America·D PandaL Wilson
Jan 1, 1997·Annual Review of Cell and Developmental Biology·A Desai, T J Mitchison
Mar 11, 2000·Molecular Cell·C DongP J Goldschmidt-Clermont
Mar 10, 2001·International Journal of Urology : Official Journal of the Japanese Urological Association·T Kitamura
Nov 21, 2002·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Rene E Harrison, Sergio Grinstein
Dec 6, 2003·Science·Anne J RidleyAlan Rick Horwitz
Jun 8, 2004·Traffic·Sandrine Etienne-Manneville
Jun 24, 2006·Nature Reviews. Cancer·Brian Bierie, Harold L Moses
Jul 25, 2008·Nature·Alberto MantovaniFrances Balkwill
Feb 11, 2010·Current Opinion in Immunology·Alberto Mantovani, Antonio Sica
Oct 20, 2010·The Journal of Pathology·Erik Meulmeester, Peter Ten Dijke
Jan 20, 2011·Neoplasia : an International Journal for Oncology Research·Jian ZhangKenneth J Pienta
Jun 29, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Margaret L NovakTimothy J Koh
Jul 30, 2011·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Jelena KocicJuan F Santibanez
Sep 29, 2011·European Journal of Immunology·Alberto MantovaniSubhra K Biswas
Sep 29, 2011·European Journal of Immunology·Christel VérolletIsabelle Maridonneau-Parini
Nov 1, 2011·Trends in Cell Biology·Katarina Wolf, Peter Friedl
Jan 25, 2012·The Journal of Biological Chemistry·Raed HananiaRene E Harrison
Apr 13, 2012·Molecular Biology of the Cell·Maria EkmanMaréne Landström
May 29, 2012·Current Pharmaceutical Design·Margherita GiganteElena Ranieri
Jul 11, 2012·Clinical & Developmental Immunology·Ning-Bo HaoShi-Ming Yang
Mar 13, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Andrew J FleetwoodJohn A Hamilton
Aug 16, 2014·Cancers·Theerawut ChanmeeNaoki Itano
Oct 16, 2015·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Kristi R ChakrabartiStuart S Martin
Feb 16, 2016·Immunobiology·Alexei Gratchev
Jan 22, 2017·Cold Spring Harbor Perspectives in Biology·Shomyseh SanjabiMing O Li
Mar 28, 2017·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·J Martin BrownSamuel Strober
Apr 18, 2017·Advances in Immunology·Aoife KellyMark A Travis
Apr 26, 2017·Oncology Reports·Takahisa YamaguchiTetsuo Ohta
May 12, 2017·F1000Research·Ivar Noordstra, Anna Akhmanova
Oct 25, 2017·British Journal of Cancer·Shweta Aras, M Raza Zaidi
Feb 17, 2018·Biochimie Open·Saroj SinghLi Yong
Mar 30, 2018·Frontiers in Oncology·Ashleigh R Poh, Matthias Ernst

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Methods Mentioned

BETA
nuclear translocation
acetylation

Software Mentioned

TScratch
NIH
ImageJ

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