Estrogen and glucocorticoid regulate osteoblast differentiation through the interaction of bone morphogenetic protein-2 and tumor necrosis factor-alpha in C2C12 cells

Molecular and Cellular Endocrinology
Yoshinori MatsumotoHirofumi Makino

Abstract

Imbalanced functions between osteoclasts and osteoblasts are involved in inflammatory bone damage. The clinical effectiveness of blocking TNF-alpha in treatment of active rheumatoid arthritis established the significance of TNF-alpha in the pathogenesis. In the present study, we investigated the cellular mechanism by which estrogen and glucocorticoid interact in osteoblastic differentiation regulated by BMP and TNF-alpha using mouse myoblastic C2C12 cells. The expression of estrogen receptors, (ER)alpha and ERbeta, and glucocorticoid receptor (GCR) was significantly increased by BMP-2 treatment regardless of the presence of estradiol and dexamethasone. Estradiol, but not dexamethasone, enhanced BMP-induced Runx2 and osteocalcin expression in C2C12 cells. In addition, TNF-alpha suppressed BMP-2-induced Runx2 and osteocalcin expression, and estradiol and dexamethasone reversed the TNF-alpha effects on BMP-2-induced Runx2 expression. Dexamethasone also abolished osteocalcin expression induced by BMP-2. Interestingly, BMP-2-induced Smad1/5/8 phosphorylation and Id-1 promoter activity were enhanced by estradiol pretreatment. On the other hand, dexamethasone suppressed BMP-2-induced Smad1/5/8 activation. TNF-alpha-induced SAPK/JNK ac...Continue Reading

References

Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·A J CelesteJ M Wozney
Jun 1, 1989·Endocrinology·M Noda, J J Camilliere
Nov 1, 1988·Cell and Tissue Research·C ManiatopoulosA H Melcher
Feb 1, 1995·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·A Herbertson, J E Aubin
Jun 1, 1994·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·E MathieuJ Merregaert
Feb 28, 1994·Biochemical and Biophysical Research Communications·V Midy, J Plouët
Feb 1, 1994·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·E Mathieu, J Merregaert
Apr 1, 1993·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·S D BainM W Edwards
Jan 1, 1997·The American Journal of the Medical Sciences·M Notelovitz
Feb 5, 1999·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·A SamuelsJ H Tobias
Jun 24, 1999·Molecular Endocrinology·T C SpelsbergS Khosla
Oct 28, 1999·Journal of Cellular Biochemistry·J P RodríguezG Seitz
Nov 24, 1999·The Journal of Clinical Investigation·L I PlotkinT Bellido
Sep 8, 2000·Molecular Endocrinology·M RazandiE R Levin
Mar 13, 2001·Annual Review of Immunology·M Feldmann, R N Maini
Sep 14, 2001·Current Opinion in Rheumatology·J A ClowesR Eastell
Nov 16, 2001·Reviews in Endocrine & Metabolic Disorders·R S Weinstein
Jun 14, 2002·The Journal of Bone and Joint Surgery. American Volume·Jay R LiebermanThomas A Einhorn
Sep 19, 2002·The Journal of Pathology·Osami KudoNicholas A Athanasou
Oct 16, 2002·The Journal of Endocrinology·T HirayamaN A Athanasou
Jun 25, 2003·Biochemical and Biophysical Research Communications·Masaya TakedaHirofumi Makino
Aug 21, 2004·Nuclear Receptor·Yasuto TaguchiDonald L Bodenner
Jan 21, 2006·Molecular and Cellular Endocrinology·Julie JacobRaghava Varman Thampan
Jul 13, 2006·Annals of the New York Academy of Sciences·Alberta Zallone
Aug 18, 2006·The New England Journal of Medicine·D L Scott, G H Kingsley
Apr 3, 2007·Biochemical and Biophysical Research Communications·Tomoyuki MukaiHirofumi Makino

❮ Previous
Next ❯

Citations

Jun 19, 2013·Molecular and Cellular Endocrinology·Ulrike BaschantJan Tuckermann
Feb 22, 2012·Biology of Sex Differences·Eric D AustinKirk B Lane
Jun 11, 2011·Pathology Research International·André BarkhordarianFrancesco Chiappelli
May 15, 2012·Trends in Molecular Medicine·Paraskevi MoutsatsouAthanasios G Papavassiliou
Oct 18, 2012·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Nan YangYan Jin
Mar 8, 2012·International Journal of Cancer. Journal International Du Cancer·Tomohiko SaseHironobu Sasano
Aug 17, 2021·Frontiers in Cell and Developmental Biology·Kai YouXuewen Xu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.